Overview on Listeria monocytogenes ecological niches, antibacterial susceptibility and virulence in bovine, caprine and ovine udder infection as a public health implication. | ||
| Veterinary Medical Journal (Giza) | ||
| Article 5, Volume 67, Issue 1, 2021, Pages 45-53 PDF (315.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2021.192304 | ||
| Authors | ||
| Nagwa Hassan1; Wageih Armanious1; Sabry Morgan2; Ahmed Samir1; Ahmed Orabi* 1 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt | ||
| 2Department of food and milk hygiene, Faculty of Veterinary Medicine, Cairo University, Egypt | ||
| Abstract | ||
| A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110), buffaloes (n = 87) ) , does (n=108) ) and ewes (n=105) from different Egyptian farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 % at apparently healthy cow , 2.77 % in subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as L.monocytogenes confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm. | ||
| Keywords | ||
| L.monocytogenes; mastitis; ruminant; sequencing; virulence; antibacterial; public health | ||
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