Raheel, I., Orabi, A., Erfan, A., Shaheen, H., Hassan, S. (2021). Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine. test, 67(1), 54-62. doi: 10.21608/vmjg.2021.192316
Ismail Raheel; Ahmed Orabi; Ahmed Erfan; Hassan Shaheen; Shaimaa Hassan. "Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine". test, 67, 1, 2021, 54-62. doi: 10.21608/vmjg.2021.192316
Raheel, I., Orabi, A., Erfan, A., Shaheen, H., Hassan, S. (2021). 'Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine', test, 67(1), pp. 54-62. doi: 10.21608/vmjg.2021.192316
Raheel, I., Orabi, A., Erfan, A., Shaheen, H., Hassan, S. Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine. test, 2021; 67(1): 54-62. doi: 10.21608/vmjg.2021.192316

Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine

Veterinary Medical Journal (Giza)
Article 6, Volume 67, Issue 1, 2021, Pages 54-62    PDF (321.63 K)
Document Type: Original Article
DOI: 10.21608/vmjg.2021.192316
Authors
Ismail Raheel1; Ahmed Orabi* 2; Ahmed Erfan3; Hassan Shaheen4; Shaimaa Hassan5
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suief University, Egypt
2Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
3Biotechnology unit, Animal health institute, Egypt
4Veterinarian in Egyptian livestock field
5Department of Microbiology, Animal Health Research Institute, Agriculture research center, Egypt.
Abstract
Meat producing broiler chickens may needs dietary amino acids of animal origin to obtain high growth, good health, muscles yield and healthy immune response. To investigate the effects of the amino acids mixture from animal origins rich in glycine on performance and immunity of broiler chickens an in vivo experiment was conducted at which T1 andT2 groups supplemented with amino acid mixture in every 10 days for successive 3 days, 8 hr. daily interval at dose 1 ml/L in drinking water and the second ( T2 group) was experimentally challenged with avian pathogenic E.coli (APEC) and the third group (T3) had no supplementation. The obtained results showed that amino acids mixture treated groups( T1 and T2) had  a remarkable immune-modulation as noticed by  significant  elevation of phagocytic count and activities(lysozymes and nitric oxide production) ,expression of  IL-4, IL-10 and  interferon- gamma-genes using RT-PCR. Performance studies proved that there was an improvement of body weight gain and  lowered mortality rate in amino acids treated group.
Keywords
Glycine amino acid; Avian E. coli; Immunomodulation; growth enhancing; Broiler chickens
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