Isolation of Salmonella species in freshly dead rabbit samples in Qalubiya Governorate, Egypt | ||
| Benha Veterinary Medical Journal | ||
| Article 32, Volume 40, Issue 2, July 2021, Pages 159-163 PDF (290.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2021.69278.1385 | ||
| Author | ||
| lamiaa abelazeam elmenyawy* | ||
| microbiology vetrinary medicine banha university | ||
| Abstract | ||
| This study was carried to examine one hundred and sixty-three different freshly dead rabbits’ corpses collected from different husbandries of rabbit rearing located in Qalubiya governorate, suffered from mortalities along the period from January, 2020 to March 2021. From each collected corpse sample, five internal organs represented by liver, kidney, spleen, lung and intestine were collected for bacteriological investigation for the presence of Salmonella species; after which serological identification of Salmonella isolates was performed then sensitivity test was applied to detect resistance to some antibiotics groups Resistance genes (AadB ,qnrS and TetA(A))are detected . Results of bacteriological examinations revealed that isolation of thirty-one different Salmonella species, where isolated from liver 7, kidney 5, spleen 10, lung 3, and intestine 6 isolates with incidence of 22.5, 16.1, 32.3, 9.6, and 19.35% respectively. Moreover, serological identification clarified that out of thirty-one isolates, 9(29.03%) strains were S. Arizonae, 5(16.12%) strains were S. Kentucky, 7(22.58%) strains were S. Enteritidis, and 8(25.80%) strains were S. Typhimurium, and 2(6.45%) strain was S. Banana. Referring to the obtained results, Salmonella was regarded as significant bacteria responsible for several morbidity and mortalities cases in rabbit farms. Antimicrobial susceptibility was applied on Salmonella strains and referred that salmonella have high resistance to streptomycin, tetracycline and quinolones group. Moreover, resistance genes (aadB, qnrs, and tetA(A) were also detecting using PCR. | ||
| Keywords | ||
| Salmonella species; bacteriological detection; serological identification; antimicrobial susceptibility and detection of resistance gene by using PCR | ||
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