Short-Term Conservation of Somatic Embryo Clusters for Date Palm (Phoenix Dactylifera l.) Cv. Amry | ||
| Annals of Agricultural Science, Moshtohor | ||
| Article 41, Volume 59, Issue 5, 2021, Pages 295-302 PDF (511.6 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/assjm.2021.194783 | ||
| Author | ||
| G.A. Baghdady* | ||
| Department of Horticulture , Faculty of Agriculture, Al-Azhar University, Nasr city ,Cairo, Egypt. | ||
| Abstract | ||
| This work aimed to develop a conservation protocol for date palm cv. Amry, an Egyptian semi dry rare cultivar. Slow growth conditions of storage studied by using low temperature at 15°C, beside the manipulation of salt strength of nutrient medium,( MS, 1/2 MS or1/4 MS) in combination with osmotic agent addition sucrose, sorbitol, or mannitol at constant concentration (0.3 M) for each, to conserve somatic embryos clusters explants, which obtained previously through direct somatic embryogenesis of inflorescences explants. Survival percentage and recovery were evaluated after 6 months of conservation period. Results showed that conserved somatic embryos clusters explants on full MS, ½ MS, conservation medium supplemented with sucrose or sorbitol recorded the highest significant survival percentage on full MS, ½ MS, where the lowest significant survival percentage was obtained when somatic embryos were conserved on 1/4 medium supplemented with mannitol . Best shoot number and shoot length recovery under normal growth conditions achieved on conservation medium supplemented with sucrose in comp inaction with reducing the full MS salt strength to the half for 6 months of conservation period under low temperature at 15°C. | ||
| Keywords | ||
| Date palm; Sorbitol; Mannitol; Sucrose; Slow growth conditions; Germplasm conservation | ||
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