COMPARISON OF PHYLOGENIC TREE AND GENE SEQUENCE OF SALMONELLA ENTERITIDIS ISOLATED FROM DIFFERENT BIRDS | ||
| Egyptian Poultry Science Journal | ||
| Article 6, Volume 41, Issue 3, September 2021, Pages 525-534 PDF (474.68 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/epsj.2021.195781 | ||
| Author | ||
| Heba Roshdy Mohamed Labib | ||
| bacteriology,Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Ministry of Agriculture. | ||
| Abstract | ||
| Salmonella is bacterium causing high morbidity and mortality rates in the birds’. The present study was performed on a total of 100 samples from diseased and apparently healthy quail came from governorates (Giza, Cairo, Damietta and kafrelsheik). Collected samples included different organs (liver, heart, lung, and bone marrow). And a comparison between the genetic tree and gene sequences of Salmonella isolated from {chicken-ducks and rabbit}. The bacteriological examination revealed that out of 100 collected samples, 22 samples (22 %) were positive for Salmonella isolation. the serotyping of Salmonella species isolates showed a major variety of serotypes which included Salmonella Enteritidis (27.3%); Salmonella Typhimurium and Salmonella Senftenberg (22.8% for- each ); Salmonella Agona, (18.1%) and Salmonella Magherafelt (9%) . Results of the sensitivity testing of salmonella isolated from quail. Showed that (72.8%, 68.2%) of strains were resistant to Nalidixic acid and Streptomycin, but sensitive to Gentamicin, Trimethoprim-sulfamethoxazole, (68.2%) of strains. Also, all Salmonella isolates showed multidrug resistance. We selected three strains to be sequenced with accession number MT267777 to MT267779 and it resembled Salmonella enterica subsp. enterica serovar Enteritidis strain from China, Korea, and UK with 100% identitiy percent. | ||
| Keywords | ||
| Salmonella enteritides; antibiotic sensitivity; sequence gene | ||
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