Farag, M., Emam, A., Mohamed, M. (2005). IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES. test, 19(2), 178-190. doi: 10.21608/fjard.2005.197844
Mostafa M. Farag; Ahmed M. Emam; Mamdouh A. Mohamed. "IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES". test, 19, 2, 2005, 178-190. doi: 10.21608/fjard.2005.197844
Farag, M., Emam, A., Mohamed, M. (2005). 'IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES', test, 19(2), pp. 178-190. doi: 10.21608/fjard.2005.197844
Farag, M., Emam, A., Mohamed, M. IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES. test, 2005; 19(2): 178-190. doi: 10.21608/fjard.2005.197844

IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES

Fayoum Journal of Agricultural Research and Development
Article 16, Volume 19, Issue 2, July 2005, Pages 178-190    PDF (791.65 K)
Document Type: Research articles.
DOI: 10.21608/fjard.2005.197844
Authors
Mostafa M. Farag1; Ahmed M. Emam2; Mamdouh A. Mohamed2
1Biochemistry Department, Faculty of Agric., Cairo Univ., Giza Egypt.
2Biochemistry Department, Faculty of Agric. Fayoum Branch, Cairo Univ., Egypt.
Abstract
The antioxidant effects of aqueous ethanolic extracts (80 %)from
the leaves of four plants i.e. Ruta chalepensis, Gomphocarpus sinaicus,
Segertia thea and Acacia holoseriaca were evaluated in vitro by
determining their effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH)
decoloration, inhibition of DNA-sugar damage and inhibition of lipid
peroxidation. Among the four extracts tested, Ruta chalepensis and
Gomphocarpus sinaicus extracts showed the strongest antioxidant
activity. The ethanolic extract of Ruta chalepensis leaves was subjected
to isolation and structural characterization of the active constituent(s) of
this plant. Two active flavonoid compounds were isolated by using a
combination of chromatographed methods (TLC and column
chromatography). Their chemical structures were characterized by
spectroscopic methods (UV, 1H and 13C-NMR) as Quercetin 3-O-
[rhamnosyl (1-6) glucoside] (I) and Isorhamnetin 3-O-glucoside-7-Orhamnoside (II).

Keywords
Natural antioxidants; Ruta chalepensis; lipid peroxidation; flavonoids; DPPH
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