ANTIBACTERIAL ACTIVITY OF FUROQUINOLONE ALKALOIDS AGAINST POTATO SOFT ROT BACTERIUM, Erwinia carotovora FROM Ruta chalepensis LEAVES | ||
| Fayoum Journal of Agricultural Research and Development | ||
| Article 17, Volume 19, Issue 2, July 2005, Pages 191-202 PDF (845.39 K) | ||
| Document Type: Research articles. | ||
| DOI: 10.21608/fjard.2005.197845 | ||
| Authors | ||
| Ahmed M. Emam1; Mohamed E. Mahmoud2 | ||
| 1Biochemistry Department, Faculty of Agriculture, Fayoum Branch, Cairo Univ., Egypt. | ||
| 2Botany Department (Microbiology), Faculty of Science, Cairo Univ., Giza, Egypt. | ||
| Abstract | ||
| Soft rot bacterium; Erwinia carotovora is one of the most severe post-harvest diseases of potatoes worldwide. This bacterium affects tubers during storage, transit and marketing. The aqueous ethanolic extract (80%) of Ruta chalepensis leaves (Rutaceae) showed antibacterial activity against Erwinia carotovora (MIC = 625 µg/ml). Bioassay-guided isolation of this aqueous extract by using a combination of different chromatographic methods (TLC and column chromatography) yielded two furoquinolone alkaloids for the first time from this plant. Their structures were characterized as 2, (hydroxy isopropyl) – 6, hydroxy –9, methyldihydrofuro [2,3] –quinol-4-one (I) and 3, (hydroxy methyl)-6, methoxy-2, 2, 9-trimethyl-dihydrofuro [2,3] quinol-4-one (II) by 1 H, 13C NMR and MS spectral data. The isolated alkaloids I and II showed antibacterial activity against Erwinia carotovora with MIC at 20 and 35 µg/ml respectively. | ||
| Keywords | ||
| Furoquinolone alkaloids; Soft rot bacterium; Ruta chalepensis; Rutaceae and Erwinia carotovora | ||
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