The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation | ||
| Egyptian Journal of Veterinary Sciences | ||
| Article 5, Volume 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, October 2021, Pages 35-40 PDF (1.39 M) | ||
| Document Type: Conference | ||
| DOI: 10.21608/ejvs.2021.90194.1264 | ||
| Authors | ||
| Aseel Kamil Hameed* 1; Jihad abdulameer Ahmed2 | ||
| 1Basic science Dept., College of Dentistry, Uni. of Basrah, Iraq | ||
| 2Dept. of Pathology, college of veterinary medicine, uni. of Basra, Iraq | ||
| Abstract | ||
| The protective role of Vit.B12 on neurotoxic effect of diazinon has been studied and conducted on 24 adults male wistar rats were divided equally into 4 groups, the group I was used as normal control; group II was orally given 1/10 LD50 (3.8mg/kg.bw) of diazinon for one month; group III was orally given 1/10 LD50 (3.8mg/kg.bw) of diazinon plus systemic injection (I.M route) of Vit.B12 (4 mg/kg.bw) for one month; group IV was given systemic injection (I.M route) of Vit.B12 (4 mg/kg.bw) only for one month. Biochemically it revealed a significant (P≤0.05) increase in GSH, CAT and SOD values in both Vit.B12 groups (group III and group IV) as well to control group when compared to diazinon group (group II); while the values of MDA and peroxynitrite revealed a significant (P≤0.05) decreases in the both Vit.B12 groups (group III and group IV) as well to control group when compared to diazinon group (group II); moreover, the histopathological results of the diazinon group (group II) revealed a serious neurologic changes included necrotized neurons, diffuse neuronal edema, perivascular edema and hyperplasia of glial cells; while the Vit.B12 groups showed no effective histopathological changes were more or less to control group. There are a little information has been provided concerning the relation between diazinon and Vit.B12,therefore, the current study concluded that the Vit.B12 has a significant protective role against the neurological toxic effects of diazinon on basis of histopathological and biochemical patterns. | ||
| Keywords | ||
| Histology; Neuron; Vitamin B12; Diazinon; Toxicity | ||
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