Enumeration and Detection of Main Pathogenic Bacterial Genera in Rahawy and Bilbeis Agriculture Drains | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 11, Volume 25, Issue 5, September and October 2021, Pages 189-202 PDF (640.96 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2021.198525 | ||
| Author | ||
| Hoda Kabary et al. | ||
| Abstract | ||
| Pathogens in agricultural drainage water represent a major threat to the public health of humans and livestock, food safety, and ecosystem quality as well. The microbiological intensities of pathogenic bacteria including, total fecal bacteria, E. coli, Salmonella sp., Shigella sp., Campylobacter sp. streptococci sp. E. coli O157 and Listeria sp., in two sites of each of Rahawy and Bilbeis agricultural drains, were characterized by selective media culturing techniques. Selective Media applied for detection and counting were MacConkey agar (MAC), Salmonella Shigella agar (SS Agar), Campylobacter Blood free selective medium (CCDA), Modified Tryptic soy agar (TSA), MacConkey sorbitol agar (SMAC), and Listeria oxford agar for total fecal bacteria, E. coli, Salmonella sp., Shigella sp., Campylobacter sp. streptococci sp. E. coli O157 and Listeria sp. count respectively. The strains were identified and selected by the reaction obtained after full growth on the differential agar media. The obtained results confirmed that Rahawy and Bilbeis water samples exhibited high intensities with pathogenic bacteria compared to river Nile water. River Nile water samples were free from either Salmonella or Shigella, with the lowest numbers of Campylobacter sp. E. coli and E. coli O157 (3x102, 30 and 1, respectively). Rahway water samples (after Hadar site) had the highest density of E. coli (5x104) and Salmonella sp. (4x103), while Bilbeis (at Bridge site) had a lower density of Salmonella (10) and Shigella sp. (20) and the highest density of Campylobacter sp. (6x104). | ||
| Keywords | ||
| Pathogenic bacteria; Agriculture drainage water; El-Rahawy drain; Bilbeis drain | ||
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