A Genetic Risk Factor in Egyptian Children with A Family History of End-Stage Renal Disease | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 8, Volume 13, Issue 2, December 2021, Pages 101-112 PDF (1.26 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2021.198981 | ||
| Authors | ||
| Ahmed Ghazy1; Manal Abdel- Salam2; Mohamed, I. Rady3; Mahmoud A. Khalifa1 | ||
| 1Bioinformatics & Molecular Biology Unit, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt | ||
| 2Nephrology Unit, Pediatric Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. | ||
| 3Cytology and Histology Lab., Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt. | ||
| Abstract | ||
| Background: Family history of end-stage renal disease (ESRD) is a significant risk factor for the subsequent development of nephropathy. Recently, several studies have shed light on single-nucleotide polymorphisms (SNPs) within the renalase gene that is believed to be associated with this disease. Objective: Investigate the association of the susceptibility of rs2296545 and rs10887800 genotypes/alleles within the renalase (RNLS) gene in children with family history of ESRD. Methods: The study included eight children on regular hemodialysis with a family history of chronic kidney disease (CKD), thirty children their ages range from 4-18 years without a family history, and 27 controls. We evaluated demographic and biochemical information, and renalase genotypes/alleles of rs2296545 and rs10887800 by the Sanger sequencing method. All data, including genotypes and alleles frequencies, were analyzed. Results: The statistical evaluation of the rs2296545 showed significant increase relatively in CC genotypes and C alleles in dialyzed patients with a family history of CKD to those without family history with odds ratio of 7.70 (95%CI=3.35-17.72) and 3.16(95% CI=1.77-5.63); respectively. In rs10887800, there was a significant increase in GC and GG genotype distribution in dialyzed patients without a family history of CKD compared to the control group, odds ratio of 2.095 (95% CI=1.0057 - 5.265) and 2.301 (1.0057 - 5.265) (1.0322 - 4.254); respectively. Conclusion: rs2296545 C allele may be considered as one of the genetic risk factors for ESRD pathogenesis in children with family history, and its corresponding G allele may have a protective role. | ||
| Keywords | ||
| Renalase; Family history of ESRD; Single nucleotide Polymorphisms (SNPs); ESRD | ||
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