Metwally, M., Mubarak, H., Gamea, A., Elzawawy, N. (2022). Isolation, Characterization and Identification of Active Antifungal Compound from the Ethanolic Leaf Extract of Pluchea dioscoridis. test, 62(1), 11-20. doi: 10.21608/ejbo.2018.955.1077
Metwally Metwally; Hanan Mubarak; Ahmed Gamea; Nessma Elzawawy. "Isolation, Characterization and Identification of Active Antifungal Compound from the Ethanolic Leaf Extract of Pluchea dioscoridis". test, 62, 1, 2022, 11-20. doi: 10.21608/ejbo.2018.955.1077
Metwally, M., Mubarak, H., Gamea, A., Elzawawy, N. (2022). 'Isolation, Characterization and Identification of Active Antifungal Compound from the Ethanolic Leaf Extract of Pluchea dioscoridis', test, 62(1), pp. 11-20. doi: 10.21608/ejbo.2018.955.1077
Metwally, M., Mubarak, H., Gamea, A., Elzawawy, N. Isolation, Characterization and Identification of Active Antifungal Compound from the Ethanolic Leaf Extract of Pluchea dioscoridis. test, 2022; 62(1): 11-20. doi: 10.21608/ejbo.2018.955.1077

Isolation, Characterization and Identification of Active Antifungal Compound from the Ethanolic Leaf Extract of Pluchea dioscoridis

Egyptian Journal of Botany
Article 2, Volume 62, Issue 1, January 2022, Pages 11-20    PDF (1.69 M)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2018.955.1077
Authors
Metwally Metwally1; Hanan Mubarak1; Ahmed Gamea2; Nessma Elzawawy* 3
1Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
2Otolaryngology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
3Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
Abstract
SECONDARY metabolites from medicinal plants have antimicrobial properties and thus are used to cure chronic and acute diseases worldwide. This study aimed to isolate and identify the antifungal compound in the ethanolic extract of Pluchea dioscoridis leaves. Qualitative and quantitative phytochemical analysis for the secondary metabolites of P. dioscoridis was assessed. Thin layer chromatography (TLC) was used. Preliminary screening for antifungal activity against otomycotic Aspergillus niger was carried out for all isolated fractions at different concentrations. Results showed that phenolic compound RF1 exhibited excellent antifungal activity against A. niger (MIC= 2mg/mL).UV spectrum, IR, HNMR, mass spectrum and high performance liquid chromatography (HPLC) analysis identified this compound as gallic acid, with a molecular formula of C7H6O5. Therefore, gallic acid is the potential antifungal compound of P. dioscoridis extract.
Keywords
Antifungal activity; Gallic acid; Pluchea dioscoridis
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