GENOTYPING AND PHENOTYPING FOR SOME BREAD WHEAT GENOTYPES UNDER TERMINAL HEAT STRESS | ||
| Egyptian Journal of Plant Breeding | ||
| Article 12, Volume 24, Issue 1, March 2020, Pages 195-223 PDF (1.23 M) | ||
| Document Type: Original research | ||
| DOI: 10.12816/ejpb.2020.199316 | ||
| Abstract | ||
| The present investigation was conducted at Shandaweel Agricultural Research Station, Agricultural Research Center, Egypt during two winter seasons to assess 12 bread wheat genotypes under normal and late sowing dates by using agronomic data and SSR markers. The studied genotypes were tested in a RCBD experiment for each sowing date; normal was on 25th November while late sowing date (heat stress) was on 25th December during 2017/2018 and 2018/2019 seasons. The results indicated highly significant differences among genotypes, sowing dates, and seasons for all traits. Significant or highly significant differences were found for the interactions between genotype x sowing date, genotype x seasons, sowing date x season, and genotype x sowing date x season for most of the studied traits. Based on heat susceptibility index the genotypes were classified into three groups i.e. tolerant, moderate tolerant, and sensitive. The molecular marker analysis showed high values of polymorphism information content (PIC) and marker index (MI). A positive and significant association was found between grain filling period and Xgwm293 marker with 87 bp specific band (r = 0.58*), as well as between grain filling rate and Xgwm557 marker with 437 bp specific band (r = 0.67*). These markers are suggested as candidate linked markers with the particular traits; they can be used in marker assisted selection (MAS). Specific fragments were unique to some genotypes. Although, some of these fragments were found to be linked with some of the studied traits, the others were not. The dendrograms successfully grouped the studied genotypes based on their performance under sowing dates as well as based on SSR patterns. The current study revealed that there were some high yielding genotypes tolerant to terminal heat stress. These genotypes can be utilized either in terminal heat stress affected regions or in breeding programs as a heat tolerance source. | ||
| Keywords | ||
| Agronomic traits; Molecular markers; Sowing dates; Grain filling period | ||
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