Dare, C., Oyedapo, O., Akinlalu, A., Komolafe, I., Fajobi, A., Ogunsusi, M. (2021). Genotoxic Activities of Polysaccharides from Cotyledon and Coat of Fermented and Unfermented Annona squamosa L. Seed. test, 12(1), 189-207. doi: 10.21608/eajbsh.2021.199654
Christianah Adebimpe Dare; Oluokun Oluboade Oyedapo; Akintunde Oluseto Akinlalu; Ige Joseph Komolafe; Adeniyi Oluwadare Fajobi; Mayowa Ogunsusi. "Genotoxic Activities of Polysaccharides from Cotyledon and Coat of Fermented and Unfermented Annona squamosa L. Seed". test, 12, 1, 2021, 189-207. doi: 10.21608/eajbsh.2021.199654
Dare, C., Oyedapo, O., Akinlalu, A., Komolafe, I., Fajobi, A., Ogunsusi, M. (2021). 'Genotoxic Activities of Polysaccharides from Cotyledon and Coat of Fermented and Unfermented Annona squamosa L. Seed', test, 12(1), pp. 189-207. doi: 10.21608/eajbsh.2021.199654
Dare, C., Oyedapo, O., Akinlalu, A., Komolafe, I., Fajobi, A., Ogunsusi, M. Genotoxic Activities of Polysaccharides from Cotyledon and Coat of Fermented and Unfermented Annona squamosa L. Seed. test, 2021; 12(1): 189-207. doi: 10.21608/eajbsh.2021.199654

Genotoxic Activities of Polysaccharides from Cotyledon and Coat of Fermented and Unfermented Annona squamosa L. Seed

Egyptian Academic Journal of Biological Sciences, H. Botany
Article 16, Volume 12, Issue 1, June 2021, Pages 189-207    PDF (2.16 M)
Document Type: Original Article
DOI: 10.21608/eajbsh.2021.199654
Authors
Christianah Adebimpe Dare1; Oluokun Oluboade Oyedapo2; Akintunde Oluseto Akinlalu3; Ige Joseph Komolafe4; Adeniyi Oluwadare Fajobi5; Mayowa Ogunsusi5
1Department of Biochemistry, Faculty of Basic and Applied Sciences Osun State University Osogbo, Osun State, Nigeria.
2Department of Biochemistry and Molecular Biology Obafemi Awolowo University Ile-Ife
3Department of Pharmaceutical Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria
4Department of Biochemistry, Hallmark University, Ijebu-Itele, Nigeria.
5Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
Abstract
This study extracted polysaccharides from cotyledon and coats of fermented and unfermented A. squamosa seed and determined the genotoxic potential of the polysaccharides. Fresh and ripe sugar apple fruits were collected from an orchard at Ota-Efun, Osogbo, Nigeria and authenticated at IFE Herbarium, Obafemi Awolowo University, Ile-Ife, Nigeria. The seeds were divided into two: a portion was fermented traditionally and the other unfermented. The cotyledon and the coat of both the fermented and unfermented seeds were separated and defatted. Polysaccharides were isolated to yield fermented cotyledon polysaccharide (FCP), fermented seed coat polysaccharide (FSCP), unfermented cotyledon polysaccharide (UCP), and unfermented seed coat polysaccharide (UFSCP). The estimation of hexoses, total hexosamines, and total uronic acid concentrations in the polysaccharide was carried out using HPLC. Genotoxic activities of the polysaccharides were investigated using standard methods.
           The analyses of the polysaccharide composition revealed that the polysaccharides were all heteropolysaccharides. Genotoxic investigations of the polysaccharides revealed very low mitotic indices, chromosomal aberration, and very low LC50.
           The study concluded that the polysaccharides from fermented and unfermented cotyledon and coat of A. squamosa seeds have excellent genotoxic activities and potential compounds in the search for drugs for combating cancer and other diseases related to oxidative stress.
Keywords
Cancer; chromosome; DNA; free radicals; oxidative stress; A. squamosa
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