Ali, M., Osman, E., Dawoud, E., Ibrahim, T., Amin, G. (2007). OPTIMIZATION OF THE BIOAGENT Bacillus subtilis BIOMASS PRODUCTION AND ANTIBOSIS AGAINST Acremonium strictum. test, 32(5), 4075-4090. doi: 10.21608/jssae.2007.201290
M.A. Ali; Ettimad A.H. Osman; Ensaf I. Dawoud; Thanaa F. Ibrahim; G.A. Amin. "OPTIMIZATION OF THE BIOAGENT Bacillus subtilis BIOMASS PRODUCTION AND ANTIBOSIS AGAINST Acremonium strictum". test, 32, 5, 2007, 4075-4090. doi: 10.21608/jssae.2007.201290
Ali, M., Osman, E., Dawoud, E., Ibrahim, T., Amin, G. (2007). 'OPTIMIZATION OF THE BIOAGENT Bacillus subtilis BIOMASS PRODUCTION AND ANTIBOSIS AGAINST Acremonium strictum', test, 32(5), pp. 4075-4090. doi: 10.21608/jssae.2007.201290
Ali, M., Osman, E., Dawoud, E., Ibrahim, T., Amin, G. OPTIMIZATION OF THE BIOAGENT Bacillus subtilis BIOMASS PRODUCTION AND ANTIBOSIS AGAINST Acremonium strictum. test, 2007; 32(5): 4075-4090. doi: 10.21608/jssae.2007.201290

OPTIMIZATION OF THE BIOAGENT Bacillus subtilis BIOMASS PRODUCTION AND ANTIBOSIS AGAINST Acremonium strictum

Journal of Soil Sciences and Agricultural Engineering
Article 11, Volume 32, Issue 5, May 2007, Pages 4075-4090    PDF (689.01 K)
Document Type: Original Article
DOI: 10.21608/jssae.2007.201290
Authors
M.A. Ali1; Ettimad A.H. Osman2; Ensaf I. Dawoud1; Thanaa F. Ibrahim2; G.A. Amin1
1Agric. Microbiology Dept., Fac. Of Agric. Cairo Univ., Egypt.
2Maize, Sugar and Forage Crops Dis. Res. Dept, Plant Pathol. Res. Inst., Agric. Res. Center, Giza
Abstract
All A. stritum strains, isolated from grain sorghum plants showing Acremonium wilt disease symptoms, varied in their virulence on both tested grain sorghum genotype with the superiority of strain No. 2. 
Among 5 different recommended media, King's medium was the most favorable one for propagation and highest antagonistic activity of Bacillus subtilis, previously isolated from rhizosphere of resistant grain sorghum cultivar (Dorado) against A. strictum.
The optimum environmental conditions needed for B. subtilis to give maximum antagonistic efficiency against A. strictum were 48h incubation period, 30-35oC incubation temperature and pH 7.0. in shake-flask submerged culture. Sucrose (9.0 g.l-1) and KNO3(20 g.l-1) were the best carbon and nitrogen sources, respectively.
Utilization of agro-industrial wastes for B. subtilis  propagation in modified King's medium showed that glucose  syrup and corn steep liquor as cheap carbon and nitrogen sources, respectively supported the highest antagonistic potential of B. subtilis  using shake-flask submerged culture.
Keywords
Bacillus subtilis; Biological control; Acremonium wilt; Acremonium strictum; Agro-industrial wastes; Antifungal activity; Optimization; Propagation
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