Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin | ||
| Egyptian Journal of Veterinary Sciences | ||
| Article 14, Volume 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, October 2021, Pages 61-68 PDF (1.39 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2021.95036.1292 | ||
| Authors | ||
| Mohamed Saleh* 1; A.M.M. El Hady1; Sahar R. Mohamed1; A. A. El-Shafei1; Maher El-Shafei2 | ||
| 1Bacteriology Department , Animal Health Research Institute, Dokki , Giza, Egypt | ||
| 2Department of Medical Microbiology Faculty of Medicine Zagazig University, Egypt | ||
| Abstract | ||
| Our study applied on 160 collected samples, from poultry and human patients. Infection percent of human skin, poultry bumble foot and human nasal swab and Diabetic foot swab were 66% , 10% , 67.5% , 37.5% respectively. Methicillin resistant Staph aureus (MRSA) were 83.3% and 50 % from poultry and human patient respectively, mec A gene is detected by 100% on samples of poultry and humen origin. Whereas blaZ and spa gene and also, 83.3% and 50% respectively either from human or poultry samples respectively. Methicillin Resistance S. aureus (MRSA) strains isolated from human nasal swabs was 80 % . PCR technique was used for mec A , blaZ ,and Spa gene detection. PCR results were identical to disc diffusion method in 11 isolates out of 15 ( 73 %) isolates from poultry and human samples. | ||
| Keywords | ||
| Methicillin resistant; S.aureus; Human; Poultry; mec A gene; blaZ gene; spa gene | ||
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