Electric pulses decrease the growth activity of Erwinia amylovora bacterium | ||
| African Journal of Biological Sciences | ||
| Article 19, Volume 17, Issue 1, 2021, Pages 261-270 PDF (501.07 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajbs.2021.201678 | ||
| Authors | ||
| Mai Ramadan Mostafa* 1; Fadel Ali1; Naglaa M. Balabel2 | ||
| 1Biophysics Department, Faculty of Science, Cairo University, Egypt | ||
| 2Plant Pathology Research Institute, Agric. Res. Center, Egypt | ||
| Abstract | ||
| The current work studies the exposure effect of Erwinia amylovora to extremely low-frequency square positive electric pulses (ELF-EP) at constant field strength of 400V/m at 28oC for various frequencies in the range 0.7-1.6 Hz. The results show that E. amylovora exposed to 1.4 Hz differs from unexposed in growth characteristic curve. The results were confirmed through DNA analysis which indicated that the change occurs only in the bacterial cell wall. Also, the results from Enterobacterial repetitive intergenic consensus (Eric-PCR), Box-PCR, Repetitive extragenic palindromic (Rep-PCR) indicated that there was no difference between unexposed and exposed bacteria to 1.4 Hz ELF-EP for different exposure durations. Therefore, it concluded that electric pulsed fields for 1.4 Hz can be used to control the growth activities of E. amylovora. | ||
| Keywords | ||
| ELF-EP; Erwinia amylovora; PCR | ||
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