Probiotic Potential of Lactobacillus plantarum Isolated from Zabady | ||
| Alexandria Journal of Food Science and Technology | ||
| Article 3, Volume 9, Issue 1, June 2012, Pages 17-31 | ||
| DOI: 10.21608/ajfs.2012.20214 | ||
| Abstract | ||
| Twenty one isolates of lactic acid bacteria (LAB) were isolated from Zabady, an Egyptian yoghurt, and screened for their antibacterial activities against 8 pathogens (Bacillus cereus ATCC 49064, Clostridium perfringens ATCC 13124, Escherichia coli, Listeria innocua ATCC 33090, Listeria monocytogenes ATCC 19116, Listeria ivanovii Li4 (pVS2), Salmonella enterica ATCC 25566, and Yersinia enterocolitica ATCC 23715) as indicator strains. One isolate was selected as a promising candidate.The selected isolate was identified as Lactobacillus plantarum by phenotypic and genotypic methods. Afterwards, the identified isolate was evaluated for a number of probiotic characteristics including tolerance to simulated gastric juice, bile salt resistance, cell surface hydrophobicity, resistance to low phenol concentration, autoggregation, coaggregation, adhesion to rabbit epithelial cells, and reduction of cholesterol. This isolate showed; sensitivity to simulated gastric juice adjusted at pH 2.0 - 3.5, tolerance to 0.3% bile concentration and antimicrobial activity against Salmonella enterica ATCC 25566 ((25600 Activity unit /ml), was Bacillus cereus ATCC 49064 (25600 Activity unit /ml), Yersinia enterocolitica ATCC 23715 (400 Activity unit /ml) and L. monocytogenes ATCC 19116 (200 Activity unit /ml) that is probably due to production of active peptides. Furthermore, the results demonstrate that this isolate was highly tolerated to different tested phenol concentrations, moderate cell surface hydrophobicity, weak autoaggregating and reasonable adhesion level to rabbit epithelial cell. | ||
| Keywords | ||
| Antibacterial activity; probiotics; lactic acid bacteria; food pathogenic bacteria; cell surface hydrophobicity | ||
|
Supplementary Files
|
||
|
Statistics Article View: 154 |
||
