Detection of Biofilm and Siderophore Encoding Genes Implicated in the Pathogenesis of Klebsiella pneumoniae Isolated from Different Clinical Specimens | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 13, Volume 30, Issue 1, January 2021, Pages 101-108 PDF (472.49 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| Author | ||
| Iman Salah Naga* | ||
| Microbiology Department, Medical Research Institute, Alexandria University, Egypt | ||
| Abstract | ||
| Background: Biofilm and siderophores protect K. pneumoniae, making them resistant to antimicrobials and increase the ability to evade immune system. Objective: The aim of the present study was to detect some of the virulence genes responsible for biofilm formation and siderophore production implicated in the pathogenesis of K. pneumoniae. Methodology: Crystal violet method was used to detect biofilm phenotypically, hypermucoviscosity by string test and virulence genes were detected by PCR. Results: Phenotypically, biofilm formation was positive in 76% and negative in 24%. Genotypically, FimH and ycfm were detected in 100% of isolates while mrkD in 98%. Kfu, entB and iutA were found in 100%, 68% and 34% respectively. Phenotypically by string test 66% of isolates were classified as hypermucoviscous, while 34% as classical. rmpA gene was detected in 52% of isolates. Conclusion: FimH, mrkD, ycfm, Kfu and iutA genes are implicated in multisystemic infection of K. pneumoniae. Enterobactin gene is more predominant than other siderophore genes. | ||
| Keywords | ||
| Klebsiella pneumoniae; Siderophores; Biofilm; Antimicrobial resistance | ||
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