Assessment of Mupirocin Resistance among Clinical Isolates of Methicillin Resistant Staphylococcus aureus | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 14, Volume 30, Issue 1, January 2021, Pages 109-114 PDF (391.4 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| Author | ||
| Nancy M. Attia* | ||
| Lecturer, Microbiology Department Medical Research Institute, Alexandria University | ||
| Abstract | ||
| Background: Methicillin-resistant Staphylococcus aureus (MRSA) colonization is considered a major risk factor for nosocomial infections and its decolonization has reduced these infections. Mupirocin (MUP) is the topical antibiotic of choice for decolonization. MUP decolonization failure is attributed to MUP resistance. Objective: The aim of the current study is to assess MUP resistance among MRSA isolates phenotypically and genotypically. Methodology: Fifty MRSA isolates were identified in Microbiology Department in the Medical Research Institute hospital, Alexandria University. Antibiotic susceptibility to different classes of antibiotics by disk diffusion method was done. MUP minimum inhibitory concentration (MIC) was determined phenotypically by MUP Ezy MIC™ Strips. MUP resistance was determined genetically by multiplex PCR detection of mupA and mupB. Results: Of all MRSA isolates, 6% exhibited high level and none showed low level MUP resistance. Only mupA was detected in all resistant isolates. Conclusion: Despite low prevalence of MUP resistance, it is appropriate to test MUP resistance prior nasal decolonization. | ||
| Keywords | ||
| Mupirocin; Resistance; Methicillin Resistant Staphylococcus aureus; Phenotypic; Genotypic | ||
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