Fregeneda-Grandes, J., El Sayed, M., Sayed, H. (2021). Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain. test, 1(2), 15-20. doi: 10.21608/nvvj.2021.203361
Juan-Miguel Fregeneda-Grandes; Mohamed R. El Sayed; Haitham H. Sayed. "Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain". test, 1, 2, 2021, 15-20. doi: 10.21608/nvvj.2021.203361
Fregeneda-Grandes, J., El Sayed, M., Sayed, H. (2021). 'Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain', test, 1(2), pp. 15-20. doi: 10.21608/nvvj.2021.203361
Fregeneda-Grandes, J., El Sayed, M., Sayed, H. Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain. test, 2021; 1(2): 15-20. doi: 10.21608/nvvj.2021.203361

Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain

New Valley Veterinary Journal
Article 3, Volume 1, Issue 2, June 2021, Pages 15-20    PDF (582.16 K)
Document Type: Original Article
DOI: 10.21608/nvvj.2021.203361
Authors
Juan-Miguel Fregeneda-Grandes1; Mohamed R. El Sayed2; Haitham H. Sayed* 3
1Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, León, España
2Department of Fish diseases and Management, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt
3Department of Microbiology, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt.
Abstract
Antibiotic resistance among fish pathogens has become a major concern. Integrons play an important role in dissemination of antibiotics resistance genes among the different species of bacteria. This study was designed to determine the antibiotic susceptibility of 20 bacterial isolates were isolated from the skin of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) collected from the rivers and fish farms in León Province, Spain and to detect class 1 and 2 integrons in these isolates by PCR to explore the role of these integrons in the antibiotic resistance. The investigated isolates included Pseudomonas fluorescens (P. fluorescens) (n=5), Aeromonas sobria (A. sobria) (n=3), Pantoea agglomerans (P. agglomerans) (n=3), Acinetobacter johnsonii (A. johnsonii) (n=2), Serratia fonticola (S. fonticola) (n=1), Staphylococcus equorum (S. equorum) (n=3), Yersinia kristensenii (Y. kristensenii) (n=2) and Xanthomonas retroflexus (X. retroflexus) (n=1). Antimicrobial susceptibility testing for the isolates revealed that they were sensitive to enrofloxacin (100%) and gentamicin (95%) while resistant to ampicillin (90%) and tetracycline (80%). PCR revealed that all the isolates didn’t harbor class 1 or 2 integrons. Therefore, the indiscriminate use of antibiotics must be restricted and more attention should be paid to biosecurity measures in aquaculture. Also, further studies are needed to assess role of integrons and other genetic elements in antimicrobial resistance in aquaculture.
Keywords
Antimicrobial; Integrons; León Province; Resistance; Trout
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