Tri Atmodjo Reubun, Y., Kumala, S., Setyahadi, S., Simanjuntak, P. (2021). Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations. test, 7(1), 31-34. doi: 10.18576/jpac/070104
Yonathan Tri Atmodjo Reubun; Shirly Kumala; Siswa Setyahadi; Partomuan Simanjuntak. "Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations". test, 7, 1, 2021, 31-34. doi: 10.18576/jpac/070104
Tri Atmodjo Reubun, Y., Kumala, S., Setyahadi, S., Simanjuntak, P. (2021). 'Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations', test, 7(1), pp. 31-34. doi: 10.18576/jpac/070104
Tri Atmodjo Reubun, Y., Kumala, S., Setyahadi, S., Simanjuntak, P. Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations. test, 2021; 7(1): 31-34. doi: 10.18576/jpac/070104

Inhibition of Acetylcolinesterase Enzyme from Pegagan Herb Extract (Centella asiatica), Kelor Leaf (Moringa oleifera) Extract and Combinations

Journal of Pharmaceutical and Applied Chemistry
Volume 7, Issue 1, January 2021, Pages 31-34    PDF (309.49 K)
Document Type: Original Article
DOI: 10.18576/jpac/070104
Authors
Yonathan Tri Atmodjo Reubun1; Shirly Kumala2; Siswa Setyahadi3; Partomuan Simanjuntak4
11Faculty of Pharmacy, Pancasila University, Lenteng Agung Srengseng Sawah, Indonesia 2Center for Bioindustrial Technology, Agency for the Assessment and Application of Technology, Indonesia 3Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Jawa Barat, Indonesia
2Faculty of Pharmacy, Pancasila University, Lenteng Agung Srengseng Sawah, Indonesia
3Center for Bioindustrial Technology, Agency for the Assessment and Application of Technology, Indonesia
4Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Jawa Barat, Indonesia
Abstract
Alzheimer's disease (AD) is a disease in which brain damage is characterized by decreased attention, memory,
and personality. AD is one result of impaired acetylcholine function. In this case, acetylcholinesterase (AChE) is an
enzyme that functions as a catalyst for the breakdown of acetylcholine (ACh) into inactive forms acetate and choline. The
purpose of this study was to determine the treatment activity of AD through AChE inhibition by Centella asiatica extract,
Moringa oleifera extract and both of combinations. The activity test was carried out based on the Ellman method wherein
this method was based on the hydrolysis ATCh substrate reaction by AChE with DTNB which gave a yellow color and
measured its absorption at a wavelength of 410 nm. The results of the AChE inhibition test showed that the combination of
pegagan herbs extract and kelor leaf extract 1: 1 gave the best inhibition with an IC50 value of 217.588 ppm. Meanwhile,
Eserin positive control with an IC50 value of 5.010 ppm. The combination of Centella asiatica extract and Moringa oleifera
extract has a synergistic effect on the treatment of AD.

Keywords
 Alzheimer; acetylcholinesterase; Centella asiatica; Moringa oleifera; Ellman’s method
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