NATURAL PRODUCTS EFFECT OF TREATMENTS ON GENE TARGETING METABOLISM ON BREAST CANCER | ||
| Journal of Productivity and Development | ||
| Article 2, Volume 27, Issue 1, January 2022, Pages 15-30 PDF (763.49 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpd.2022.205853 | ||
| Authors | ||
| Salwa Mohamed* 1; Midaa Ashry2; Khalil Halfawy3; Wael Roshdy4; Wael Abdel-Mageed5 | ||
| 1Molecular Cell Biology & Molecular Biology Department, Genetic Engineering and Biotechnoloy Research Institute,(GEBRI)University of Sadat City, Egypt. | ||
| 2Medical Laboratories Department, Faculty of Applied Medical Sciences, October 6 University, Egypt. | ||
| 3Molecular Genetics and Molecular biology Department, Genetic engineering and Biotechnology Research Institute, Sadat City University, Egypt. | ||
| 4Central Public Health Laboratories, Ministry of Health and Population, Cairo, Egypt. | ||
| 5Genetics Department, Faculty of Agriculture, Beni-Suif University, Egypt | ||
| Abstract | ||
| Breast cancer is among the most common malignant tumors. It is the second leading cause of cancer deaths among women. While mutation in the BRCA1 and BRCA2 genes confer risk of developing breast cancer. Insulin-like growth factors (IGFs) IGF-1 and IGF-2 are associated with the development and progression of breast cancer. Curcumin is a polyphenol natural product isolated from the rhizome of Curcuma longa. For centuries, curcumin has been used in medicinal preparations and as a food colorant. In recent years, Curcumin, has been reported to have anticancer and chemoprevention effects on breast cancer. Particularly, curcumin has been recognized as an effective anticancer agent that regulates multiple intracellular signaling pathways. This study is designed to investigate the effect of curcumin on gene targeting metabolism on breast cancer. To achieve this aim we conducted the study on MCF-7 cell lines, and evaluate the cytotoxicity of different concentration of curcumin by MTT assay, RNA extracted from MCF-7 cell lines by Qiagen method and reverse transcriptase to cDNA finally quantification of the expression of genes (IGF-1, IGF-2 and BRCA1) by Real time PCR (RT-PCR). The results showed thatdifferent concentration of curcuminmakeinactivation for genes related to breast cancer (IGF1, IGF2 and BRCA1) and can stop the breast cancer pathway. Conclusively, breast cancer is among the most common malignant tumors. It is the second leading cause of cancer mortality among women in the world. Curcumin, an active derivative from turmeric, has been investigated to have anticancer and chemoprevention effects on breast cancer.in this study different concentrations of curcumin influence the expression of gene targeting metabolism on breast cancer and make inactivation to it which may lead to stopping the breast cancer pathway. Our data demonstrated that curcumin could be used as the treatment of breast cancer. | ||
| Keywords | ||
| Breast cancer; IGFs genes; BRCA genes; Curcumin | ||
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