Multi Drug Resistant Pseudomonas aeruginosa in a Health Care Setting in Alexandria | ||
| Journal of High Institute of Public Health | ||
| Article 8, Volume 40, Issue 2, April 2010, Pages 333-347 PDF (191.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jhiph.2010.20608 | ||
| Author | ||
| Amany Abaza* | ||
| Microbiology Department, Alexandria University Students' Hospital, Egypt | ||
| Abstract | ||
| Background: The emergence of Multi drug resistant Pseudomonas aeruginosa (MDRPA) among intensive care unit (ICU) patients is increasingly recognized as a public health threat worldwide. Objective: This work aimed to study the occurrence of MDRPA among critically ill patients in a health care setting in Alexandria. Methods: During a 12 months period, different clinical samples (sputum, endotracheal aspirates, blood, urine, and pus) obtained from ICU patients were tested for the isolation and identification of Pseudomonas aeruginosa (P. aeruginosa) strains; that were screened for their antimicrobial susceptibility patterns using single disc diffusion method. Identified MDRPA strains were further tested for their susceptibility to polymyxin E (colistin), polymyxin B, and tigecycline. Polymerase chain reaction (PCR) assay was performed to detect VIM and IMP MBL genes. Results: Of the 105 P. aeruginosa strains isolated from various clinical samples, 20 (19%) were found to be MDRPA, of which 16 (80%) were sensitive to each of colistin and polymyxin B, while only 5 (25%) strains were sensitive to tigecycline. PCR assay revealed that 9 (45%) strains possessed VIM MBL gene and none (0%) harbored IMP MBL gene. Conclusion: The occurrence of MDRPA strains among critically ill patients in this study was noticeable; with colistin and polymyxin B being effective upon the majority of identified MDRPA strains, and VIM MBL gene was found to be significantly harbored. | ||
| Keywords | ||
| Multidrug Resistance Pseudomonas aeruginosa; Metallo Beta Lactamases; Polymyxins; Colistin; Tigecycline; VIM gene, IMP gene; MBL gene | ||
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