Isolation, Purification and Identification of CFP29 from Mycobacterium tuberculosis H37Rv culture filtrate proteins | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Article 14, Volume 13, Issue 2, December 2021, Pages 189-198 PDF (586.89 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2021.206175 | ||
| Author | ||
| Mansoor Alsahag | ||
| Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia | ||
| Abstract | ||
| Tuberculosis (TB) continues to be a serious worldwide health issue. Human tuberculosis (TB) is the commonest cause of mortality from one infectious agent, with eight million new cases and two million fatalities each year. In many animal models of TB, proteins isolated from the culture filtrate of Mycobacterium tuberculosis promote protective immunity. The extracellular proteins of Mycobacterium tuberculosis were isolated, purified and identifiedin the current study. M. tuberculosis H37Rv was culturedin Souton’s medium and the extracellular proteins were isolated employing an ion-exchange column chromatography before their purification and characterization using SDS- PAGE, western blotting and N- terminal sequencing. CFP29 was identified and purified in M. tuberculosis H37Rv culture filtrate. However, further characterization of this protein is needed to be utilized as an effective T-cell antigen produced from culture filtrates. | ||
| Keywords | ||
| Isolation; Purification; CFP29; Mycobacterium tuberculosis; culture filtrate proteins | ||
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