PHYTOCHEMICAL STUDY AND PROTECTIVE EFFECT INVESTIGATION AGAINST OXIDATIVE DAMAGE IN HUMAN ERYTHROCYTES OF SYRIAN ASTERACEAE PLANTS | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 5, Volume 44, Issue 2, December 2021, Pages 323-338 PDF (738.73 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2021.207152 | ||
| Authors | ||
| M.Kinan Aljaja* 1; Raghda Lahdo2; Adawia Kitaz2 | ||
| 1Department Of Pharmacognosy and medicinal plants, Faculty of Pharmacy, University of Aleppo, Syria | ||
| 2Department Of Biochemistry and Microbiology, Faculty of Pharmacy, University of Aleppo, Syria | ||
| Abstract | ||
| Oxidative stress is a major cause of many diseases, it can damage the tissues and lead to lipid peroxidation and DNA strand breakage. Nowadays, researchers have centered medicinal plants in their concern, to benefit from their active component as antioxidant reagents. This study aims to investigate the protective and hemolytic activity, as well as qualitative and quantitative chemical content of plants from Syrian Flora. Ethanol 70% extracts of three plants of Asteraceae; Onopordum carduiforme, Centaurea verutum, and Achillea santolina were prepared to test their protective antioxidant effect on erythrocytes membrane against the oxidative hemolysis which was induced using AAPH. The hemolytic effect of these plants was also evaluated. In addition to the quantitative content of phenols TPC and flavonoids TFC compounds using Folin-Ciocalteu and aluminum chloride colorimetric methods respectively. The basic phytochemical screening was conducted using the standard methods.The results showed a low hemolytic effect of the extracts on erythrocytes, using the concentrations of (1-5 mg/ml). Moreover, they reduced membrane lipid peroxidation significantly; where O. carduiforme showed the highest efficacy of protective activity against hemolytic damage. The phytochemical screening of the plants’ extracts showed phenols, flavonoids, tannins, coumarins, cardiac glycosides, terpenoids, steroids, and carbohydrates presence, whereas no alkaloids or saponins were found. TPC and TFC content ranges were between (9.22 ± 0.412 and 13.716 ± 0.431 mg GAE/g DW) and (2.224 ± 0.346 and 8.958 ± 0.216 mg RUE/g DW) respectively. | ||
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