CYTOTOXIC EFFECT OF SILVER NANOPARTICLES ON COLORECTAL ADENOCARCINOMA CELL LINE | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 25, Volume 44, Issue 2, December 2021, Pages 587-597 PDF (1.01 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2021.207188 | ||
| Authors | ||
| Alaa Salman* 1; Chadi Soukkarieh2, 3; Samar Alzeer4 | ||
| 1department of Toxicology, faculty of pharmacy, Damascus university, Damascus, Syria | ||
| 2Department of Biology, Faculty of Sciences, Damascus University, Damascus, Syria | ||
| 3Center for Epidemiological and Biological Studies of Leishmania Parasites, Damascus University, Damascus, Syria | ||
| 4Department of Toxicology, Faculty of Pharmacy, Damascus University, Damascus, Syria | ||
| Abstract | ||
| Silver nanoparticles (Ag-NPs) with antimicrobial activity are by far the most commercialized nano-compound. However, there is limited information about antitumor potential. Therefore, the present study was aimed to observe the effect of small-dimensional Ag-NPs (20 Nm) on human epithelial colorectal adeno-carcinoma (Caco-2) cells. Caco-2 cells were exposed to Ag- NPs with different concentrations (1–15 μg/mL) for 24 and 48 hrs. and cytotoxicity was analyzed by MTT assay and IC50 was calculated. In addition, flow cytometry was used to determine the effect of Ag-NPs on the cell cycle progression and on apoptosis. An MTT assay revealed that following exposure to Ag-NPs the viability of Caco-2 cells decreased, and the viability decreased further with increasing exposure time. The IC50 was 7.85 μg/ml and 4.92 μg/ml at 24 and 48 hrs. respectively after exposure. The flow cytometry technique revealed that Ag-NPs induced a significant increase in the percentage of cells in G1 phase of the cell cycle and of apoptotic cells. In conclusion, Ag-NPs exhibited dose- and time-dependent cytotoxicity, and these results have signposted that Ag-NPs were promising nanoparticles which could be a potential candidate in treating colorectal cancer. | ||
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