EFFECT OF MEDIUM COMPOSITION ON IN VITRO PROPAGATION OF Oxalis deppei. | ||
| Journal of Plant Production | ||
| Article 24, Volume 32, Issue 4, April 2007, Pages 2895-2905 PDF (578.91 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpp.2007.207691 | ||
| Author | ||
| H. A. A. Hussein* | ||
| Veget. and Flor. Dept., Faculty of Agriculture, Mansoura University. | ||
| Abstract | ||
| The present research was carried out to study the effect of 4 types of culture media containing various mineral salts of 4 media types on the in vitro micropropagation of Oxalis plant. Each medium was supplemented with vitamins as described by Murashige & Skoog (1962) casein hydrolysate(1.0 g/l), sucrose (30 g/l), Difco agar (7.0 g/l), GA3 (0.1 mg/l) and certain growth regulators at different concentrations on proliferation and multiplication of bulb explants. Bulbs at a small size (0.8 – 1.5 cm long and 0.5 – 0.8 thickness) excised from mother plants grown in the greenhouse, were surface sterilized and cultured on the proliferation medium. The developed bulblets were excised and cultured on multiplication medium to produce small plantlets. The obtained results indicated that the mineral salts of Murashige & Skoog (1962) which supplemented with vitamins as described by M & S (1962), casein hydrolysate (1.0 g/l) sucrose (30.0 g/l), Difco agar (7.0 g/l), casein hydrolysate (1.0 g/l), GA3 (0.1 g/l), 6-benzylaminopurine (BAP) at 0.3 mg/l and Indole-3-acetic acid (IAA) at 0.3 mg/l showed significant positive effect on leaves and bulblets proliferation expressed as highest survival percentage of cultured explants, leaves initiation was occurred within a few days, highest percentage of explants which formed leaves and highest number of leaves and bulblets per explant. The obtained results showed also that culture medium containing the mineral salts of M & S (1962) and vitamins as described by M & S (1962) at full strength which supplemented with casein hydrolysate (1.0 g/l), sucrose (30.0 g/l), Difco agar (7.0 g/l), GA3 (0.1 mg/l), BAP at 0.3 mg/l and IAA at 0.3 mg/l yielded better results of the growth vigor of bulb explants expressed as highest survival percentage, leaves initiation was occurred within a few days and highest number of leaves as well as bulblets per explants than vitamins free medium and those containing M & S vitamins at half strength. In addition, Murashige & Skoog (1962) medium enriched with casein hydrolysate (1.0 g/l), sucrose (30.0 g/l), Difco agar (7.0 g/l), GA3 (0.1 mg/l), kinetin (1.0 mg/l) and BAP (0.1 mg/l) was the most favourable medium for leaves and bulblets proliferation as well as the highest survival percentage. Leaves initiation was occurred within a few days, the highest percentages of explants which formed leaves and the number of leaves and bulblets per explant. Moreover, the multiplication medium was the Murashige & Skoog (1962) medium containing casein hydrolysate (1.0 g/l), sucrose (30.0 g/l), Difco agar (7.0 g/l), GA3 (0.1 mg/l), kinetin at 0.5 mg/l and BAP at 0.05 mg/l which produced the highest survival percentage; leaves initiation was occurred within a few days, the highest percentage of explants which formed leaves; the highest number of leaves and bulblets and the highest number of roots as well as the longest roots per explant. Rooted bulblets were successfully transferred to a mixture of peat moss and perlite (3 : 1 v/v) and kept under intermittent mist for one week in the greenhouse to complete their normal growth. | ||
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