Mycological and Molecular detection of some fungi causing diarrhea in sheep and goats | ||
| Benha Veterinary Medical Journal | ||
| Article 17, Volume 41, Issue 1, October 2021, Pages 78-83 PDF (368.04 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2021.82448.1439 | ||
| Authors | ||
| Ashraf Abd El Tawab1; Fatma Elhofy2; Eman Moustafa Moustafa Moustafa* 3; Ramadan Tag Eldin4; Enas Soliman5; Khaled Gebril5 | ||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University | ||
| 2Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt | ||
| 3Egypt, Kafr El-Sheikh University, Faculty of Veterinary Medicine, Fish Diseases and Management Department | ||
| 4Chief researcher of mycology and mycotoxin, Animal Health Institute – Dokki– Giza | ||
| 5Bacteriology, Immunology and Mycology Dep., Fac. of Vet. Med., Benha Univ. | ||
| Abstract | ||
| Sheep and goat are important production sources of meat and milk in Egypt. Diarrhea is very common and the chronic form of it may lead to deaths, so the accurate diagnosis of fugal diseases causing diarrhea is a priority. In the current study, the fungal infections causing diarrhea in sheep and goats were investigated. From 200 examined samples; 27 yeast isolates (13.5%) and 112 mold isolates (56.00%) were recovered from the mycological examinations of the collected samples from diarrheic animals, contact workers and feed stuffs. A. flavus and Rhodotorula sp. were the most prevalent mould and yeast isolated from the examined samples, respectively. A. flavus and Rhodotorula sp. were molecularly identified using PCR tests. They showed clear bands at 305 bp molecular weight for A. flavus and 560bp for Rhodotorula sp. PCR is a useful method for diagnosing fungi that cause diarrhoea in sheep and goats in a direct and timely manner. | ||
| Keywords | ||
| diarrohea; goat; mould; PCR; sheep | ||
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