Incidence and Duplex PCR for detection of S.aureus and L.monocytogenes in Meat Products | ||
| Benha Veterinary Medical Journal | ||
| Article 23, Volume 41, Issue 1, October 2021, Pages 106-110 PDF (949.97 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2021.78579.1426 | ||
| Authors | ||
| George Armany* 1; Hemmat Moustafa Ibrahim2; Reham Abdel Aziz Amin2; Naglaa Hussein Hagag1 | ||
| 1Genome Research Unit-Animal Health Research Institute | ||
| 2Food Hygiene Dept. (Meat Hygiene) Faculty of Veterinary Medicine-Benha University | ||
| Abstract | ||
| One hundred 100 random samples of raw meat products (minced meat and sausage, 25 of each) and treated meat products (luncheon and basterma, 25 of each) were gathered from various markets in the provinces of Cairo and Giza for a period of 3 months. Accurately, 25 samples from each product were gathered from various markets in the provinces of Cairo and Giza. Traditional bacteriological methods and duplex polymerase chain reaction were used detect S. aureus and L. monocytogenes in such examined samples. Actually, S. aureus was isolated from 24%, 28%, 16% and 12% in minced meat, sausage, luncheon and basterma, respectively. Only, L. monocytogenes was detected in 4% of minced meat. Characterization of S. aureus by (nuc) gene and L. monocytogenes by (hlyA) gene using duplex Polymerase Chain Reaction. Concerning duplex PCR results, the incidence of S.aureus in minced meat, sausage, luncheon and basterma was (24%, 24%, 16%, 8%), respectively. While, the incidence of L. monocytogenes in minced meat, sausage, luncheon and basterma was (4%, 0%, 0%, 0%), respectively. Duplex PCR showed a great agreement with the results of bacteriological method which proves the utility of the molecular technique in detection of food pathogens. | ||
| Keywords | ||
| S. aureus; L. monocytogenes; nuc gene; hlyA gene; duplex PCR | ||
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