Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes | ||
| Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology | ||
| Article 4, Volume 13, Issue 2, December 2021, Pages 35-44 PDF (598.38 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbse.2021.209694 | ||
| Author | ||
| Mansoor Alsahag | ||
| Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia | ||
| Abstract | ||
| Resuscitation promoting factor E (RpfE) proteins in Mycobacterium tuberculosis (M. tuberculosis) may be recognized by the host immune system owing to their secretory nature. In this study, the RpfE protein was purified using DEAE–Sepharose column chromatography from M. tuberculosis H37Rv extracellular proteins. An upregulated production of gamma interferon (IFN- γ), interleukin-10 (IL-10) and IL-12 by splenocytes suggest T cell proliferation stimulated by purified RpfE protein. The present findings imply that the RpfE protein produced extracellularly in M. tuberculosis H37Rv can be used as a viable subunit vaccine candidate. | ||
| Keywords | ||
| Mycobacterium tuberculosis; RpfE; Purification; Culture filtrate proteins; Splenocytes | ||
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