BARGHASH, S., TAHA, S., SERAG, S., RAGAB, E. (2021). IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT. test, 51(3), 617-626. doi: 10.21608/jesp.2021.210453
SAFAA M. BARGHASH; SAMIR A. TAHA; SARA S. A. SERAG; EHAB A. RAGAB. "IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT". test, 51, 3, 2021, 617-626. doi: 10.21608/jesp.2021.210453
BARGHASH, S., TAHA, S., SERAG, S., RAGAB, E. (2021). 'IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT', test, 51(3), pp. 617-626. doi: 10.21608/jesp.2021.210453
BARGHASH, S., TAHA, S., SERAG, S., RAGAB, E. IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT. test, 2021; 51(3): 617-626. doi: 10.21608/jesp.2021.210453

IDENTIFICATION AND GENOTYPES OF GIARDIA INTESTINALIS IN RUMINANT LIVESTOCK IN SOUTH SINAI GOVERNORATE, EGYPT

Journal of the Egyptian Society of Parasitology
Article 22, Volume 51, Issue 3, December 2021, Pages 617-626    PDF (1.08 M)
DOI: 10.21608/jesp.2021.210453
Authors
SAFAA M. BARGHASH1; SAMIR A. TAHA2; SARA S. A. SERAG3; EHAB A. RAGAB4
1Parasitology Unit, Department of Animal and Poultry Health, Animal Production and Poultry Division, Desert Research Center
2Department of Zoology, Department, Faculty of Science, Zagazig University
3Faculty of Science, Zagazig University
4Agricultural Economics Institute
Abstract
This study may be the first to detect genotype of Giardia among ruminants in South Sinai Governorate.
Fresh iodine-fecal stained smears were examined by microscopy, followed by a nested
PCR on frozen fecal specimens. The results showed Giardia in 40% (78/195) & 28.72%
(56/195) of the examined ruminants by microscopic and nPCR, respectively. In cattle was
(52.94%, 39.2%), in goats (37.5, 25.0%), and in sheep (34.82%, 25.0%). Animals <3 months old
were more Giardia infected than older ones. Giardia infection was high in El Tur City (50%,
35.1%) than in Ras Sudr City (25.9%, 19.8%), and in males (51.3%, 35.5%) than in females
(32.8%, 24.4%). Symptomatic animals were more susceptible to the infection than asymptomatic
ones. Giardia was more prevalent in diarrheic samples than those with soft and hard nature with
significant differences (P<0.05). Nested-PCR of G. intestinalis identified positive β-giardin gene
band of 511 & 753bp. Analysis of five sharp generated fragments in both directions showed sequences
with accession numbers MW925063, MW925066, & MW925067 (Assemblage A) &
MW925064 & MW925065 (Assemblage B). It clustered with some Kenyan and Egyptian isolates
with identity from 93.9% to 100% and genetic distance from 0.0 to 6.5 compared to other
GenBank strains. Genotyping at sub-Assemblage level was essential for zoonotic transmission.
Keywords
Sinai; Giardia intestinalis; nested-PCR; beta-giardin (bg); Gene sequencing; Ruminants
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