The impact of addition of ascorbic acid to cryopreservation medium on dog epididymal spermatozoa | ||
| Veterinary Medical Journal (Giza) | ||
| Article 14, Volume 67, Issue 1, 2021, Pages 161-173 PDF (306.77 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/vmjg.2021.210948 | ||
| Authors | ||
| Eman Fayez1; Ali Salama* 1; Zaher M Rawash2; Mohamed A. I. El Sayed1 | ||
| 1Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt | ||
| 2Animal Reproduction Research Institute, Giza, Egypt | ||
| Abstract | ||
| The objective of this study was to assess the effect of the addition of different concentrations of ascorbic acid to the extender on frozen-thawed epididymal dog spermatozoa. Epididymides from 17 castrated dogs were minced and incubated in a Tris buffer. The retrieved spermatozoa were diluted with Tris based-egg yolk-glycerol extender supplemented with different concentrations of ascorbic acid (0.45 mg/ml and 0.90 mg/ml) and the control (0.0 mg/ml). Diluted samples were equilibrated at 5°C for 2 h, packaged in 0.25 ml straws, and stored in liquid nitrogen (-196°C). After thawing (37°C for 30 s), sperm motility, viability, membrane integrity, acrosome integrity, DNA integrity, and lipid peroxidation by malondialdehyde (MDA) concentration were evaluated. The results were expressed as mean ± SE. Adding 0.90 mg/ml ascorbic acid to the cryopreservation medium significantly (P<0.05) improved motility, viability, membrane and acrosome integrity compared to the control. MDA concentration was significantly (P<0.05) reduced at 0.90 mg/ml related to the control. Percent of DNA damage was significantly (P<0.05) reduced in 0.45 mg/ml and 0.90 mg/ml ascorbic acid compared to the control. In conclusion, addition of ascorbic acid (0.90 mg/ml) to TCF extender resulted in a significant increase in the percentage of motility, viability, membrane intact, and acrosome- intact canine epididymal sperm, as well as the maintenance of DNA integrity and the reduction of lipid peroxidation at the membrane level. | ||
| Keywords | ||
| Dog; Epididymal sperm; Cryopreservation; Ascorbic acid; oxidative stress | ||
|
Statistics Article View: 299 PDF Download: 199 |
||
