Microbiological and genetical identification of some vermicompost beneficial associated bacteria | ||
| SVU-International Journal of Agricultural Sciences | ||
| Article 3, Volume 4, Issue 1, January 2022, Pages 21-36 PDF (887.89 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/svuijas.2021.106875.1154 | ||
| Authors | ||
| H.A.H. Soltan* 1; O.F. Dakhly2; M.A. Mahmoud2; Ysamen F.M. Fayz2 | ||
| 1Central Laboratory of Organic Agriculture, Agriculture Research Center, 12619 Giza, Egypt | ||
| 2Genetics Department, Faculty of Agriculture, Minia University, 61519 Minia, Egypt | ||
| Abstract | ||
| n the present work, isolation and identification of some plant beneficial bacteria of vermicompost were conducted. A total of ten bacterial isolates were selected and characterized morphologically and biochemically. Molecular characterization of the bacterial isolates using 16S rRNA gene sequence analysis and phylogenic tree were conducted. According to the percentages of homology of 16S rRNA sequence with the closest NCBI strain, all of the ten bacterial isolates were belonged to six species (B. subtilis, B. atrophaeus, B. hemicentroti, B. velezensis, B. nakamurai and B. amyloliquefaciens) of genus Bacillus. Except isolate YFV05 which showed only 99.77% of homology with Bacillus subtilis strain NRRL NRS-744, all of the other isolates showed 100% homology to different strains of Bacillus spp < /em>. Generally, results revealed that all the ten bacterial isolates had different levels of in vitro antagonistic effect against four phytopathogenic fungi (Fusarium solani, Fusarium spp, Macrophomina phasolenia, and Rhizoctonia solani). In addition, treatment with YFV014, YFV05 and YFV012 isolates offered the highest values of vigor index (2166.0, 1775.0 and 1733.6), respectively. Finally, it could be concluded that plant beneficial bacteria might be isolate from vermicompost which in turn can be used to improve plant growth directly by production of plant growth-regulating hormones and enzymes and indirectly by controlling plant pathogens and other pests, thereby enhancing plant health and minimizing the yield loss of various crops. | ||
| Keywords | ||
| 16S rRNA; Bacillus spp; Gene sequencing; Vermicompost | ||
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