Spectrophotometric Determination of Metronidazole in Pharmaceutical Preparations and in Human Blood samples | ||
| Egyptian Journal of Chemistry | ||
| Article 36, Volume 65, Issue 8, August 2022, Pages 397-405 PDF (571.85 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.112958.5134 | ||
| Authors | ||
| Nada Ahmadd khalil1; Hana Shuker Mahmoud* 2; Ahlam ahmad Shehab3 | ||
| 1Collage of pharmacy/University of Mosul | ||
| 2Department of chemistry/college of science/university of Mosul | ||
| 3College of Education for Pure Sciences University of Mosul | ||
| Abstract | ||
| This research investigates a sensitive quantitative estimation of metronidazole (MZOL) in each of its pharmaceutical preparations and its level in human blood with one color reaction and one simple spectrophotometric technique. Metronidazole the antibiotic and antiprotozoal medication is determined by the reduction reaction of MZOL to 2-(2-Methyl-5-amino-1H-imidazole-1-yl) ethanol, followed by coupling with diazotized p-amino benzophenone (PABPh) reagent. The produced color complex is measured at 431 nm. The proposed method is successfully applied for the determination of metronidazole in different dosage forms (tablet, suspension, and intravenous injection) with high precision (RSD% from ±0.011 to ±2.3). as well as it applied for the determination of metronidazole in human blood samples of healthy voluntaries easily and accurately after 4, 6, 12, and 24 hours of oral administration with relative standard deviation not more than ±1.922 which provide the ability for therapeutic monitoring follow up of metronidazole in blood. | ||
| Keywords | ||
| Metronidazole; spectrophotometry; pharmaceutical preparations; blood samples | ||
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