Anti-Virulence and Cytotoxic Behavior of Copper Nanoparticles against antibiotics-resistant Staphylococcus aureus | ||
| Egyptian Journal of Chemistry | ||
| Article 34, Volume 65, Issue 9, September 2022, Pages 361-367 PDF (846 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.111832.5079 | ||
| Authors | ||
| Amr A. El-Waseif* 1; Hind A. Eissa2; Sabah A. Abo El Maaty2; Mervat G. Hassan2 | ||
| 1Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt. | ||
| 2Botany and Microbiology Dept., Faculty of Science, Banha University, Egypt. | ||
| Abstract | ||
| Staphylococcus aureus is one of the most common and distributed pathogens. S. aureus was resistant to 11 antibiotics including four categories based on their functions. Synthesis of copper nanoparticles (CuNPs) from CuSO4.5H2O using ascorbic acid was performed. Properties of CuNPs using UV and TEM were measured. Results of characterization observed absorption peak at 592 nm and CuNPs cubic in shape with average size 3.5 nm. The activity of CuNPs as bactericidal agent against S. aureus was determined using agar well diffusion method. CuNPs showed greater antibacterial activity and MIC was 40 μg/ml. The anti-biofilm activity of CuNPs was assayed in 96-well polystyrene plates and 3 ml polyethylene tube. Obviously, remarkable progressive inhibition of biofilm formation was noticed with increasing of CuNPs concentrations. Determination of CuNPs cytotoxicity on WI-38 cells using MTT protocol was performed. The toxicity observed at concentration 62.5 was 23% with IC50 value 134.65 μg ml−1. | ||
| Keywords | ||
| Keywords: CuNPs; Staphylococcus aureus; bactericidal; Biofilm; cytotoxicity | ||
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