Zakaria, M., El Sayeh, H., Abdel El Azem, M., El-Dereny, S. (2021). Role of the Date Palm Pollen Grains (Phoenix dactylifera L.) in Induced Antimicropial Peptides Synthesis and Improved the Royal Jelly Protein Properties in Honey Bees. test, 12(12), 857-860. doi: 10.21608/jppp.2021.220025
M. E. Zakaria; Heba Allah S. El Sayeh; M. A. I. Abdel El Azem; Sarah H. El-Dereny. "Role of the Date Palm Pollen Grains (Phoenix dactylifera L.) in Induced Antimicropial Peptides Synthesis and Improved the Royal Jelly Protein Properties in Honey Bees". test, 12, 12, 2021, 857-860. doi: 10.21608/jppp.2021.220025
Zakaria, M., El Sayeh, H., Abdel El Azem, M., El-Dereny, S. (2021). 'Role of the Date Palm Pollen Grains (Phoenix dactylifera L.) in Induced Antimicropial Peptides Synthesis and Improved the Royal Jelly Protein Properties in Honey Bees', test, 12(12), pp. 857-860. doi: 10.21608/jppp.2021.220025
Zakaria, M., El Sayeh, H., Abdel El Azem, M., El-Dereny, S. Role of the Date Palm Pollen Grains (Phoenix dactylifera L.) in Induced Antimicropial Peptides Synthesis and Improved the Royal Jelly Protein Properties in Honey Bees. test, 2021; 12(12): 857-860. doi: 10.21608/jppp.2021.220025

Role of the Date Palm Pollen Grains (Phoenix dactylifera L.) in Induced Antimicropial Peptides Synthesis and Improved the Royal Jelly Protein Properties in Honey Bees

Journal of Plant Protection and Pathology
Article 6, Volume 12, Issue 12, December 2021, Pages 857-860    PDF (625.08 K)
Document Type: Original Article
DOI: 10.21608/jppp.2021.220025
Authors
M. E. Zakaria; Heba Allah S. El Sayeh; M. A. I. Abdel El Azem; Sarah H. El-Dereny*
Bee Research Deptartment, Plant Protection Research Institute, Agriculture Research Center, Ministry of Agriculture, Dokki, Giza, Egypt.
Abstract
Date Palm Pollen Grains (Phoenix dactylifera L.) (DPPG) were used by feeding honey bee colonies to study their role in raising immunity peptides and improving the Royal Jelly (RJ) protein properties. The Haemolymph and RJ of adult nurse honey bee workers following the feeding process at two concentrations (10 & 30%) were collected. Total protein and Electrophoretic protein analysis (SDS–PAGE) were done. From the results obtained, it could be concluded that treated honey bee colonies by the DPPG led to increased total Haemolymph protein numbers, particularly of the lower molecular weights with both tested concentrations. 2-The RJ protein molecular weights were reduced from (240 kDa) (maximum molecular weights in the control) to (71 kDa).  3-The RJ at (10%) conc. of the feeding process showed four proteins ≤ 14 kDa, among them known as Hymenoptaecin (12 kDa), Defencin (4.8 kDa), and Apidaecin (2 kDa). While with the feeding conc. (30%), it recoded one of the AMPs only, Apidaecin (2 kDa). 4–The RJ in its normal state (as a control) contains no proteins with molecular weights less than 14 kDa. So, it could be recommended that beekeepers feed their bee colonies with DPPG, particularly at the concentration (10%) that allows them to increase the antimicrobial peptides in the RJ, which is healthy nutrition for both the bees and people consumption. 
Keywords
Pollen Grains; Date Palm; Royal Jelly; Honeybee; Haemolymph; Immunity
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