Possible Effects of Mirabegron on Rat Liver in Renal Ischemia Reperfusion Injury: Histological and Immunohistochemical Studies | ||
| Minia Journal of Medical Research | ||
| Volume 31, Issue 3, July 2020, Pages 308-316 PDF (1.28 M) | ||
| DOI: 10.21608/mjmr.2022.220309 | ||
| Authors | ||
| Mahmoud M. Montaser; Ibrahim . K K Ragab; Esam O. Kamel | ||
| Department of Medical Histology and Cell Biology, Faculty of Medicine, Al-Azhar University in Assuit | ||
| Abstract | ||
| Liver and kidney are both critical controller organs in our body and any liver or kidney damage including organ ischemia, may affect the other. Renal ischemia reperfusion injury diminishes antioxidant protein and increases neutrophils and lymphocytes amassing in liver tissue. Mirabegron is a medication used to treat overactive bladder. It works by activating the β3 adrenergic receptor in the bladder, resulting in its relaxation. We aimed in this study to investigate the possible histological and immmunohistochemical effects of mirabegron on the rat liver in cases of induced renal ischemia reperfusion injury. Fifteen rats were used and randomly classified into 5 groups, 10 rats each. Control group in which the rats received the vehicle; CMC without any surgical procedures. Sham group in which the rats received the vehicle then undergone laboratomy without ligation of renal pedicle. Mirabegron group in which the rats received 0.3mg/kg mirabegron without surgical procedures. Renal Ischemia Reperfusion group (RIR), rats administered the vehicle, after which ischemia induced (30 min) followed by reperfusion (3 hours). Mirabegron + RIR group in which the rats pretreated with 0.3 mg/kg mirabegron then RIR induced using the same procedures as that for the RIR group. The liver tissues from all groups were collected and then processed for histological and iNOs immunohistological studies.Liver sections from RIR rats showed loss of normal architecture of hepatic lobules in the form of congested central vein, restricted inflammatory cell infiltration and dilated hepatic sinusoids. Most of hepatocytes looked vacuolated with eccentric nuclei and others showed pyknotic dense nuclei. In comparison, pretreating the RIR rats with mirabegron showed restoration of the normal architecture of the hepatic lobules to great extent. Positive iNOS immunostaining in hepatocytes of RIR rats was noticed and pretreating the RIR rats with mirabegron reversed this positivity. We can conclude that mirabegron has a protective role on the rat liver in induced renal ischemia reperfusion injury. | ||
| Keywords | ||
| Liver; RIR; Mirabegron; iNOs | ||
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