Effect of Amlodipine and l-Carnitin Separately and Collectively on Certain Body Parameters that Are Related to Bone Metabolism in Ovariectomized Albino Rats | ||
| Minia Journal of Medical Research | ||
| Volume 31, Issue 2, April 2020, Pages 228-240 PDF (885.74 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjmr.2022.221022 | ||
| Authors | ||
| Ahmed F. Abdel Ghany1; Yasser M. Ashour2; Nawal B. Aly3; Lobna A. Abdelzaher4; Ahmed S. Mahmoud5 | ||
| 1Department of Physiology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt | ||
| 2Department of Physiology, Egyptian Russian University, Egypt. | ||
| 3* Department of Physiology, Egyptian Russian University, Egypt. | ||
| 4Department of Pharmacology, Faculty of Medicine, Assuit University, Egypt. | ||
| 5Department of Histology, Faculty of Medicine, Minia University, Egypt. | ||
| Abstract | ||
| Background: Amlodipine as calcium channel blocker has anti inflammatory through over augmentation of nitric oxide production, Anti oxidant effect by reduced superoxide radicals and increased the level of superoxide dismutase L-Carnitine has anti inflammatory through reacts with acyl group that accumulated in anoxic cells, Anti oxidant effect decreasing the superoxide anion production. Objective: Assessing the effect of Amlodipine and L-Carnitine Separately And Collectively in osteoporosis induced by ovariectomy in female albino rats. Materials and Methods: Fifty adult female albino rats of local strain were randomly divided into five groups of 10 animals each. G1: Negative control group that received no treatment G2: Postive control group Ovariectomized rats that will be received no treatment G3: Amlodipine treated group Ovariectomized rats that will be received Amlodipine (5mg/kg/day) dissolved in distilled water by intraperitoneal injection (after two months from ovariectomy operation ) for three weeks. G4: L-Carnitine treated group Ovariectomized rats that will be received L-Carnitine (100 mg/kg/day) by intra-peritoneal injection (after two months from ovariectomy operation ) for three weeks. G5: Amlodipine + LCarnitine Treated Group Ovariectomized 10 rats that will be received both Amlodipine at dose (5mg/kg/day) and L-Carnitine at a dose of 100 mg/kg/ day by intraperitoneal injection (after two months from ovariectomy operation) for three weeks. All rats were sacrificed and blood was withdrawn for biochemical examinations of Estrogen, Osteocalcine, Tumor necrosis factor α (TNFα) and Alkaline phosphatase (ALP). Femurs were removed for histopathological examination. Results: The serum levels of Osteocalcin, TNFα and ALP were increased significantly in positive control group as compared to negative control group. Treatment with Amlodipine and L-Carnitine for three weeks after two months from ovareiectomy operation significantly decreased the serum levels of Osteocalcin, TNFα and ALP when compared to positive control group. These results suggest that amlodipine and L-Carnitine have protective role in osteoporosis induced by ovariectomy in female albino rats. | ||
| Keywords | ||
| Amlodipine; L-Carnitine; Osteoporosis; Ovariectomized rats | ||
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