Effect of piperazine citrate on experimentally cryptosporidium parvum infected mice | ||
| Benha Medical Journal | ||
| Article 233, Volume 39, Special issue (Academic), September and October 2022, Pages 168-182 PDF (869.55 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bmfj.2021.104543.1510 | ||
| Authors | ||
| Samia Mostafa Rashed1; Maysa Ahmad Eraky2; Rabab Elsayed Omar2; kawkab Ahmed3; Asmaa Fahmy Saad* 4; Marwa Mohamed Nageeb2 | ||
| 1Parasitology Department- Faculty of Medicine- Benha University | ||
| 2parasitology department faculty of medicine benha university | ||
| 3Pathology Department, Faculty of Veterinary Medicine, Cairo University | ||
| 4Department of Medical Parasitology-Faculty of Medicine-Benha University | ||
| Abstract | ||
| Background : Nitazoxanide has not been found to be effective in (Acquired Immunodeficiency Syndrome) patients. Piperazine-derivative MMV665917 may potentially be used to treat human cryptosporidiosis; however, further investigations are required. Purpose: The aim of this work was to evaluate the effect of piperazine citrate on Cryptosporidium parvum infected mice. Methods: Stool samples would be collected from patients attending Abu-Elrish hospital , showing Cryptosporidium parvum oocyst. One hundred and thirty mice, were selected from (Theodor Bilharz Research Institute ). Half of them would take (dexamethasone) for 15 day before infection. Both Nitazoxanide (100mgkg) and piperazine citrate at different doses ( 20 , 30, 40, mgkg) were given to the mice. Assessment of drugs effect was done by parasitological and histopathological assessment . Results: the highest reduction in mean oocysts number of Cryptosporidium parvum was achieved by group treated with combination(nitazoxanide+ piperazine ) therapy for 1 week in immunocompromised group (95%) and immunocompetent group (89%) and remarkable, regression of histopathological alterations was noticed in ileum of immunocompromised mice treated with combination, when compared with ileum of immunocompromised (positive control) group which showed remarkable histopathological changes exhibited as shortening of the intestinal villi, hyperplasia of goblet cells, inflammatory cells infiltration in the lamina propria , submucosa and edema in the submucosa , multiple Cryptosporidium oocyst . Conculsion: Administration of (piperazine 30+ nitazoxanide) for 7days in immunocompromised group gave the highest reduction rate of mean number of oocysts and remarkable, regression of histopathological alterations. | ||
| Keywords | ||
| Cryptosporidium; nitazoxanide; piperazine citrate | ||
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