Nassef, N., Lebda, F., El Agaty, S., Atef, M. (2022). Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats. test, 42(2), 131-143. doi: 10.21608/besps.2021.90723.1109
Noha AbdelAziz Nassef; Fatma Lebda; Sahar El Agaty; Marina Atef. "Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats". test, 42, 2, 2022, 131-143. doi: 10.21608/besps.2021.90723.1109
Nassef, N., Lebda, F., El Agaty, S., Atef, M. (2022). 'Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats', test, 42(2), pp. 131-143. doi: 10.21608/besps.2021.90723.1109
Nassef, N., Lebda, F., El Agaty, S., Atef, M. Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats. test, 2022; 42(2): 131-143. doi: 10.21608/besps.2021.90723.1109

Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats

Bulletin of Egyptian Society for Physiological Sciences
Article 1, Volume 42, Issue 2, April 2022, Pages 131-143    PDF (632.23 K)
Document Type: Original Article
DOI: 10.21608/besps.2021.90723.1109
Authors
Noha AbdelAziz Nassef* 1; Fatma Lebda2; Sahar El Agaty1; Marina Atef3
1Physiology Department, Faculty of Medicine, Ain Shams University
2Department of Physiology, Faculty of Medicine, Ain Shams University,Cairo
3Department of Physiology, Faculty of Medicine, Ain Shams University, Cairo
Abstract
Background: Oxidative stress and inflammation are primarily implicated in the development and progression of liver injury during cholestasis. Selenium, a known essential antioxidant trace element, was found to provide a remarkable antioxidant and anti-inflammatory effects on various diseases.
Aim: This study was planned to evaluate the possible protective effect of selenium supplementation in a rat model of chronic cholestasis.
Design: Experimental study.
Methods: This study was carried out on adult male rats allocated randomly into sham, 4 weeks bile duct ligated (BDL), and BDL-selenium treated (BDL-Se) groups. Sodium selenite was given by gavage daily, in a dose of 100 µg/kg for 6 weeks, starting 2 weeks before the BDL.
Results:
BDL group presented a significant increase in serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and liver levels of malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and transforming growth factor beta 1(TGF-β1), associated with a significant decrease in serum levels of total proteins (TP) compared to sham group . Selenium supplementation significantly lowered serum levels of AST, ALT, ALP, and liver levels of MDA, TNF-α, and TGF-β1, along with a significant increase in serum TP in BDL-Se group versus BDL rats. Histological analysis of liver showed a significant attenuation of the inflammatory score and a significant decrease in the percentage area of collagen deposition in BDL-Se group versus BDL rats.
Conclusion: Selenium supplementation reduces liver injury and improves liver functions in experimental cholestasis probably by its antioxidant and anti-inflammatory activities, which further alleviate the liver fibrosis.
Keywords
Cholestasis; selenium; oxidative stress; inflammation; fibrosis
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