Genetic Diversity Among Varieties and Hybrid Lines of Pea (pisum sativuml.) as Revealed by Morphological Traits and SSR Markers | ||
| Egyptian Journal of Botany | ||
| Article 2, Volume 55, Issue 1, December 2015, Pages 17-29 PDF (725.21 K) | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2015.222 | ||
| Abstract | ||
| GENETIC diversity among five pea varieties and 20 hybrid lines was investigated based on variation in 33 morphological traits and polymorphism created by 19 SSR primers. Measurements of morphological traits clearly indicated that hybrids generally have more vigor compared to their parents. The analysis of genetic diversity using the NTSYS-pc and the CAP software produced UPGMA, NJ and CAP trees of similar topologies. In all trees, Var. Sugarless and its hybrids were separated as one group, Var. Master B and most of its hybrids as another group and Var. Lincoln, Var. Little Marvel and Var. Luxer and some of their hybrids were distinguished as different groups. Close distance was particularly found between Var. Sugarless and the hybrids Sugarless x Master B, Sugarless x Lincoln, Sugarless x Little Marvel, Sugarless x Luxer, and Luxer x Sugarless and Lincoln x Sugarless. On the other hand, low distance was observed between Var. Master B and the hybrids Master B x Lincoln, Master B x Little Marvel, Master B x Luxer, Little Marvel x Master B and Luxer x Master B. These findings may be used to guide future breeding of pea genotypes into commercial lines. | ||
| Keywords | ||
| pea; Pisum sativum; genetic variation; molecular markers; ISSR; Plant breeding | ||
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