AMELIORATIVE ROLE OF VITAMIN A ON LIVER TOXICITY INDUCED BY AFLATOXIN B1 ON ALBINO RATS: HISTOLOGICAL AND ULTRASTRUCTURAL STUDY | ||
| Egyptian Journal of Zoology | ||
| Article 5, Volume 69, Issue 69, June 2018, Pages 73-88 | ||
| Document Type: Original Research Papers | ||
| DOI: 10.12816/0049663 | ||
| Author | ||
| Amany A. Osman* | ||
| Department of Zoology, Women College for Arts, Science and Education, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| Aflatoxins are highly toxic, mutagenic, teratogenic and carcinogenic compounds produced by some species of Aspergillus, especially Aspergillus flavus and Aspergillus parasiticus. The aim of this study was to investigate the possible ameliorative role of vitamin A (Vit. A) on aflatoxin (AF) induced hepatotoxicity through histopathological and ultrastructural approaches on liver tissue of female albino rats treated with aflatoxin B1 (AFB1). Animals were divided into 5 groups of 6 rats each. Group I, animals of this group had been kept as normal without any treatment and were considered as controls. Group 2, animals of this group were orally administered vehicle 50% DMSO (dimethylsulfoxide) alone. Group 3, animals of this group were orally administered vehicle with Vit. A (132 IU double the human therapeutic dose). Group 4, animals of this group were orally administered 0.05μg AFB1 per kg dissolved in 50% DMSO. Group 5, animals of this group were orally administered 0.05μg AFB1 per kg with Vit. A. The experiment lasted for 14 weeks, animals were dissected 24 hours post treatment. The histological investigations of the treated group clearly demonstrated, active Kupffer cells, inflammation, fatty changes with many apoptotic changes. Other investigated fields, illustrated necrotic area in the portal zone, dilated and congestion blood vessels, and hydropic vacuolization of degenerated hepatocytes. At the ultrastructural level, irregular nuclear envelop, deformed endoplasmic reticulum and mitochondria were demonsterated. On the other side, the administration of Vit. A to AFB1-treated animals revealed more or less normal hepatocytes, inflammation of the portal area and well-defined central vein with scattered inflammed cells, with lobulated nuclei. So, special care must be taken when using stored grain as food, then natural sources should be used as vitamin A as an antioxidant. | ||
| Keywords | ||
| Histological; Ultrastructural; Aflatoxin B1; vitamin A; Liver; female rats | ||
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