High Prevalence of Staphylococcal Enterotoxins (SEs) Virulence Genes Among Methicillin-Resistant Staphylococcus aureus Isolated from Patients with Different Clinical Manifestations in Khartoum State, Sudan | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Article 7, Volume 14, Issue 1, June 2022, Pages 77-83 PDF (551.44 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2022.226663 | ||
| Authors | ||
| Elsadig M. Hamdan1; Asmaa A. Hassan2; Safa A. Amin3; Hiba A. Ahmed4; Amany E. Ataelmanan1; Mogahid M. Elhassan5 | ||
| 1Department of Medical Microbiology, College of Medical Laboratory Science, University of Gezira, Sudan. | ||
| 2Department of Dermatology Sudan Medical Specialization Board. | ||
| 3Department of Molecular Genetic and Bioinformatic, central laboratory, ministry of higher education and scientific research, Sudan. | ||
| 4Department of Medical Microbiology, Medical Laboratory Science program, Alnahda College, Sudan. | ||
| 5Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, PO Box 344, Al Madenah Al Monawarah, Saudi Arabia. - Department of Medical Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Sudan. | ||
| Abstract | ||
| Background: Staphylococcal enterotoxins (SEs) are family members of more than 20 different staphylococcal and streptococcal exotoxins. SEA and SEB are the most common toxins in staphylococcus-related food poisoning. Objectives The present study aimed to estimate the prevalence of SEs Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from different clinical samples. Methods: A cross-sectional laboratory-based study was conducted from November 2020 to January 2021. To isolate and identify S. aureus, the conventional method and protein A latex test were used. MRSA was detected using methicillin (MET 5μg) by using the Kirby-Baur disk diffusion method. Extracted DNA was amplified for the specific Staphylococcal enterotoxins (SE) gene to confirm the SEA, SEB and SEC. Result: The SEA gene was observed among 120 (100%) clinical isolates. The combined staphylococcal enterotoxin genes A, B and C were found in 33.3% of the isolates. Respiratory tract infection (RTI) shows the highest percentage of SEA 28.3%. Conclusion: The present study showed a high prevalence of SEA in clinical settings at Khartoum State. A high prevalence of the combination of more than one gene occur in SEA with SEC, which was represented 82.5%. The present study showed a strong relationship between MRSA and SEA that could be due to a specific strain of MRSA carrying the SEA gene. Further studies should be done about Staphylococcal enterotoxins. | ||
| Keywords | ||
| S. aureus; MRSA; RTI; PCR; SEA | ||
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