Microbiological study of some commonly used foods and drinks in Tanta Al Gharbia Governorate | ||
| Microbes and Infectious Diseases | ||
| Article 30, Volume 3, Issue 4, November 2022, Pages 1032-1043 PDF (404.92 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2022.126007.1255 | ||
| Authors | ||
| Mai Abd Elkarim1; Eman E. Hegazy2; kareman Ahmed Eshra* 2; Afaf Sayed Ahmed Zamzam2 | ||
| 1Forensic lab doctor in DNA unit in Tanta Department ,the Egyptian Forensic Medicine Authority | ||
| 2Medical Microbiology and Immunology Department, Tanta University, Faculty of Medicine, Egypt. | ||
| Abstract | ||
| Background:Food borne illness represents a major public health problem associated with high morbidity and mortality rates. Methods: One hundred samples (foods and drinks) were collected in sterile stomacher bags, Bacterial isolation, colony count, antimicrobial susceptibility testing, and detection of Staphylococcus aureus (S.aureus) enterotoxin genes by multiplex PCR were done. Results: The current study revealed that the Gram-negative bacilli were the most frequent isolated organisms representing (63.3%) followed by Gram positive bacteria (34.5%) and fungi were the least found (2.2%). Among Gram negative bacilli, Klebsiella was the most frequently isolated bacteria, among Gram positive bacteria, Staphylococci were the most frequently isolated bacteria, all isolated fungi were Candida species. Out of 16 identified S. aureus strains recovered from the examined samples, 6 isolates were proved to be enterotoxigenic. One isolate carried Staphylococal enterotoxin C (SEC) gene, two isolates carried both Staphylococcal enterotoxin A (SEA) genes and Staphylococcal enterotoxin B (SEB) gene, and three isolates carried SEA, SEB, and SEC genes. None of the isolates contained SED gene. Conclusion: Based on the findings of this study, food borne illness represent a major public health problem in Tanta. | ||
| Keywords | ||
| Keywords: contamination of food and drink; Foodborne ilness; enterotoxins; multiplex PCR | ||
|
Statistics Article View: 394 PDF Download: 263 |
||
