Shugaa Addin, A., Talaat, R., Sakr, M., Khalifa, M., Ahmed, E., Nasr, G., El Hamshary, M. (2022). Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians. test, 18(1), 29-38. doi: 10.21608/ajbs.2022.230601
Amr M. Shugaa Addin; Randa M Talaat; Moustafa A Sakr; Mohamed K Khalifa; Ehab A Ahmed; Ghada M Nasr; Manal O El Hamshary. "Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians". test, 18, 1, 2022, 29-38. doi: 10.21608/ajbs.2022.230601
Shugaa Addin, A., Talaat, R., Sakr, M., Khalifa, M., Ahmed, E., Nasr, G., El Hamshary, M. (2022). 'Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians', test, 18(1), pp. 29-38. doi: 10.21608/ajbs.2022.230601
Shugaa Addin, A., Talaat, R., Sakr, M., Khalifa, M., Ahmed, E., Nasr, G., El Hamshary, M. Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians. test, 2022; 18(1): 29-38. doi: 10.21608/ajbs.2022.230601

Next-generation sequencing (NGS) detection of PIK3CA somatic mutations in Hepatocellular carcinoma (HCC) Egyptians

African Journal of Biological Sciences
Article 3, Volume 18, Issue 1, June 2022, Pages 29-38    PDF (872.01 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2022.230601
Authors
Amr M. Shugaa Addin* 1; Randa M Talaat2; Moustafa A Sakr3; Mohamed K Khalifa2; Ehab A Ahmed4; Ghada M Nasr3; Manal O El Hamshary3
1Molecular diagnostics and therapeutics department,GEBRI.University of Sadat city
2Children Cancer Hospital 57357, Egypt
3Department of Molecular Diagnostic and Therapeutic, Genetic Engineering & Biotechnology Research Institute, University of Sadat City, Egypt
4Chemistry department, Faculty of science, Cairo University, Egypt.
Abstract
Hepatocellular carcinoma (HCC) is the tertiary greatest communal malignant cancer that origins mortality globally. A high prevalence of return means that even while cancer is getting more treatable in its early stages, advanced cases have a bad prognosis. To properly treat HCC, it is now necessary to understand its pathogenic process and its associated genetic abnormalities. The next-generation sequencing (NGS) was used to discover PIK3CA somatic mutations in HCC Egyptian patients. In the present study a unique NGS panel (AmpliSeq) containing the PIK3CA gene was utilized to examine 21 liquid biopsy samples from patients with HCC. The results indicated that over 40 -single-nucleotide-variation (SNV) were recognized in PIK3CA gene with incidence of 85.7%. The changes were dispersed between deleterious, undefined significance, and tolerated deviations, where the preponderance of the changes were the missense variants (68%),synonymous(25%), and (7%) for stop-gained. It was concluded from this research that detection of numerous somatic mutations of PIK3CA can assist in the etiology of HCC.
Keywords
PIK3CA; HCC; Non-Synonymous; Mutation; SNV; NGS
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