Hepatoprotective effect of fennel and tiger nut on biochemical parameters and DNA damage in rats | ||
| Egyptian Journal of Chemistry and Environmental Health | ||
| Volume 1, Issue 1, November 2015, Pages 18-37 PDF (506.19 K) | ||
| Document Type: Scientific and Research | ||
| DOI: 10.21608/ejceh.2015.231368 | ||
| Authors | ||
| Hanan M Sobhy* 1; Sohair S Ahmad2; Hanan A Azoz1; Areej A Yassin2 | ||
| 1Biochem .Toxicol. &Feed Deff. Dep. - Animal Health Research Institute | ||
| 2Nutrition &food science Dep.- Faculty of Home Economics Al –Azhar | ||
| Abstract | ||
| The present study was carried out to investigate studies of dried fennel and tiger nut in normal and on carbon tetrachloride (CCl4) induced DNA damage using one concentration of each plants alone for 30 days in male albino rats. A total of thirty male albino rats (150-200 g) were used in this study.Rats were divided into 6 groups each of 5 animals. Group one was kept as a control –ve and fed on basal diet only, while the two other groups were fed on basal diet mixed with grind dried fennel and tiger nut at concentration (5 g / 100 g diet) for 30 successive days. Other three groups were subcutaneous injected of CCl4 (0.1 ml/100 g b.wt. for two weeks) to induced DNA damage change .One of these group was left as a control +ve (subcutaneous injection of CCl4), where the other two groups were fed on basal diet mixed with grind dried fennel and tiger nut at concentration (5 g / 100 g diet) for 30 successive days. At the end of experimental period, blood samples were collected from each rat for biochemical analysis and rats were then sacrificed to elucidate DNA damage in hepatocytes.Subcutaneous injection of CCl4 caused significant increase in serum levels of AST,ALT,ALP,creatinine,urea, triglyceride,glucose,total cholesterol,LDL and lipid peroxide (MDA) while the levels of serum total protein, albumin ,globulin , A : G ratio and glutathione transferase (GST) were significantly decreased .The smear on agarose gel had been observed in CCl4 treated groups indicating random DNA fragmentation and a hallmark of necrosis . Fennel and tiger nut significantly restored the serum levels of biochemical parameters directed toward normal as compared with the control +ve group (injected with CCl4 ). | ||
| Keywords | ||
| Biochemical; Nutrition | ||
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