Comparison between two different DNA extraction methods to obtain high DNA quality from Astacus leptodactylus | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Article 32, Volume 26, Issue 2, March and April 2022, Pages 489-494 PDF (403.04 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2022.231706 | ||
| Author | ||
| Mehrnoush Aminisarteshnizi | ||
| Abstract | ||
| Most aquatic studies are performed at the molecular level, which needs reliable, quick DNA extraction protocols. Extraction of genomic DNA with high quality is one of the basic needs of molecular genetics. This investigation indicated two different methods for obtaining a sufficient quality of genomic DNA of crayfish (Astacus leptodactylus). The DNA from A. leptodactylus was extracted using extraction methods Quick-DNA™ Tissue/Insect Miniprep Kit and Chelex method. We used eight samples of A. leptodactylus from the Anzali lagoon for this comparison. Total DNA was isolated from the abdominal tissues of all samples. Quantitative and qualitative parameters were measured using a spectrophotometer. The results showed that the highest quality of extracted DNA was in the Kit (1.82-1.9). The Chelex method showed the existence of proteins in the samples (1.48-1.73). In both methods, DNA was isolated, but the Kit method was more trustable and applicable for having good quality DNA. | ||
| Keywords | ||
| DNA extraction; Astacus leptodactylus; spectrophotometer; Anzali lagoon | ||
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