Khalil, L., Azzam, A., Mohamed, H., Aboueldahab, M., Taha, H., Soliman, M. (2016). IN VITRO EFFECTS OF THE STEM EXTRACTS OF THE PLANT CALOTROPIS PROCERA ON SCHISTOSOMA MANSONI ADULT WORMS. test, 66(66), 217-230. doi: 10.12816/0034719
Lotfia M. M. Khalil; Ahmad M. Azzam; Hatem A. M. Mohamed; Marwa M. Aboueldahab; Hoda A. Taha; Mohammad I. Soliman. "IN VITRO EFFECTS OF THE STEM EXTRACTS OF THE PLANT CALOTROPIS PROCERA ON SCHISTOSOMA MANSONI ADULT WORMS". test, 66, 66, 2016, 217-230. doi: 10.12816/0034719
Khalil, L., Azzam, A., Mohamed, H., Aboueldahab, M., Taha, H., Soliman, M. (2016). 'IN VITRO EFFECTS OF THE STEM EXTRACTS OF THE PLANT CALOTROPIS PROCERA ON SCHISTOSOMA MANSONI ADULT WORMS', test, 66(66), pp. 217-230. doi: 10.12816/0034719
Khalil, L., Azzam, A., Mohamed, H., Aboueldahab, M., Taha, H., Soliman, M. IN VITRO EFFECTS OF THE STEM EXTRACTS OF THE PLANT CALOTROPIS PROCERA ON SCHISTOSOMA MANSONI ADULT WORMS. test, 2016; 66(66): 217-230. doi: 10.12816/0034719

IN VITRO EFFECTS OF THE STEM EXTRACTS OF THE PLANT CALOTROPIS PROCERA ON SCHISTOSOMA MANSONI ADULT WORMS

Egyptian Journal of Zoology
Article 16, Volume 66, Issue 66, December 2016, Pages 217-230
Document Type: Original Research Papers
DOI: 10.12816/0034719
Authors
Lotfia M. M. Khalil1; Ahmad M. Azzam2; Hatem A. M. Mohamed1; Marwa M. Aboueldahab1; Hoda A. Taha1; Mohammad I. Soliman1
1Faculty of Science, Ain Shams University, Cairo, Egypt
2Theodor Bilharz Research Institute, Giza, Egypt
Abstract
In vitro activity of aqueous and alcoholic extracts of the plant Calotropis procera (6.25, 12.5 and 25 mg/L) have been applied against Schistosoma mansoni adult worms. The mortality of adult worms was observed after exposure to aqueous and ethanolic stem extracts of C. procera during a period of 48 hours. The ethanolic extract of C. procera (at 25 mg/L) caused death of all worms after only half an hour. However, the concentration of 12.5 and 6.25 mg/L caused death of all tested worms after three hours of exposure to the ethanolic extract. On the other hand, the exposure of Schistosoma mansoni adult worms to the aqueous extract of C. procera (at 25 mg/L) caused death of these worms after four hours. In aqueous stem extracts, a dose of 6.5 and 12.5 decreased the worm viability in comparison with control. Moreover, in all concentrations of both aqueous and alcoholic extracts, coupled adult worms were separated into individual males and females. Alterations of male worms' tegument exposed to ethanolic and aqueous extracts in comparison with control worms were examined by scanning electron microscopy. In conclusion, C. procera alcoholic extract has a more schistomicidal activity than aqueous extract. In addition, it has been found that C. procera extract induces marked effects on tegumental surface architecture.
Keywords
Calotropis procera extracts; <i>In vitro</i> study; Schistomicidal activity; Schistosoma mansoni
Statistics
Article View: 233