Occurance of hydratic cyst in camels (Camelus dromedarius) and their effect on meat quality | ||
| Egyptian Journal of Chemistry and Environmental Health | ||
| Volume 2, Issue 1, May 2016, Pages 48-63 PDF (677.56 K) | ||
| Document Type: Scientific and Research | ||
| DOI: 10.21608/ejceh.2016.232409 | ||
| Authors | ||
| Amer R. Abdel Aziz* 1; Rasha A. El Meghanawy2; Fatma F. M. Ibrahim3; Dina I. El Zahaby4 | ||
| 1Department of parasitology, Faculty of Veterinary Medicine, Souhag University, Souhag, Egypt | ||
| 2Department of parasitology, Animal Health Research Institute (AHRI), Shebin El koom, Menoufiya,Egypt | ||
| 3Department of Biochemistry, Animal Health Research Institute (AHRI), Shebin El koom, Menoufiya, Egypt | ||
| 4Department of food hygiene, Animal Health Research Institute (AHRI), Shebin El koom, Menoufiya, Egypt | ||
| Abstract | ||
| Hydatidosis is caused by Echinococcus granulosus which is one of the important parasitic disease in warm blooded vertebrates and occasionally in many other mammal species. Their were no enough informations available regarding haematological profile, serum enzyme activity, chemical changes, and mineral profile in one humped camels (Camelus dromedaries) from Egypt with pulmonary hydatid cyst. The objective of the present study was to investigate the changes in complete haematological profile and also changes in some biochemical parameters in serum samples, such as; Total Protein, Albumin, Globulin, A/G ratio, serum liver enzyme activity such as; Alanine Amino Transaminase (ALT), Aspartate Amino Transferase (AST), bilirubin, glucose, cholesterol, also, kidney function activity as; Urea, Uric acid, and Creatinine, also, the level of complete Minirals profile, such as; Calcium, Inorganic phosphorus, Magnesium, Sodium, and Potassium were also done. Meat chemical composition of healthy and infected camels with Echinicoccus granulosus, was also made as nutrition value, proteins, fats, ashes, mineral elements, as, calcium level in meat samples was measured and compared to samples from healthy camels at abattoir, a total of Fifty samples of meat, serum and hydatid cysts from lungs were collected from camels admitted to slaughter houses at Qalyubia Governorate, Egypt. Results concluding that; meat from the camel infected with echinococcosis is of poorer quality as compared with muscle tissue of the healthy animal as well as being biologically inferior, there were a significant increase in leucocytic count, neutrophils and eosinophils while the other haemograms are non significant. More over significant increase in total protein, and globulin in sera of infected camel with hydatidosis when compared with the non infected ones. Decreased in A/G ratio reflected the changes observed in albumin and globulin fractions. A significant elevation of the activity of AST, ALT and bilirubin level (p<0.05), but a significant decrease in glucose value, and there is no alteration in serum cholesterol, urea, uric acid and creatinine level was observed. Concerning calcium and inorganic phosphorus levels, they were significant decrease, and this study showed non significantly changed in sodium, magnesium and potassium levels. On the other hand; in the present study, DNAs were extracted from protoscolices and/or associated germinal layers of hydatid cysts using a commercial kit. The (18 SSU r RNA gene) was used as targets for polymerase chain reaction (PCR) amplification. PCR products were purified and partial sequences were generated. Sequences were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from known strains of EG circulating globally. The phylogenetic analysis showed that 98% (n = 49) of the isolates clustered with Echinococcus canadensis genotype 6 (G6). Conclusions: It is clear that activity of the specific enzymes of serum and the haematological, chemical and biochemical profile can be helpful in diagnosis of pulmonary hydatid cyst infection of one humped camels. | ||
| Keywords | ||
| Echinococcus granulosus; (18SSUr-RNAgene); hematological; biochemical; Phylogenetic analysis; Egypt | ||
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