Direct organogenesis from shoot tip of Egyptian New Valley date palm cultivars | ||
| Egyptian International Journal of Palms | ||
| Volume 1, Issue 1, January 2021, Pages 124-138 PDF (448.76 K) | ||
| Document Type: Researches | ||
| DOI: 10.21608/esjp.2021.233545 | ||
| Authors | ||
| Mona M. Hassan* 1; Amal F. M. Zein El Din2; Ibrahim Shams El Din1; Ibrahim Ibrahim3; Rania Taha4 | ||
| 1The Central Laboratory of Date Palm Researches and Development, Agricultural Research Center, Giza,12619, Egypt. | ||
| 2The Central Lab. For Date palm Researches and Development, Agricultural Research Center (ARC) | ||
| 3Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt. | ||
| 4Pomology Department, Agriculture, and Biology Research Division, National Research Centre (NRC), 33 Elbohouth St., Dokki, Giza, 12622, Egypt. Tissue Culture Technique Lab., Central Laboratories Network, NRC. | ||
| Abstract | ||
| Shoot tip explants of superior date palm cultivars Sewi, Tamr El wady, Hegazi and one individual unknown female from El-Kharga city (called Faleg or Meghel) were grown in the New Valley region and cultured on three different starting culture media MS+10mg/l 2,4-D+3.0mg/l 2iP ( M1), MS+ 10mg/l NAA+ 3.0mg/l 2iP (M2) and MS+30 mg/l NAA+20mg/l 2iP(M3) for eight months (two months interval). All culture media were supplemented with 40g/l sucrose, 2.0 g/l PVP, and 3.0 g/l activated charcoal and solidified with gelrite at 2.0 g/l. After eight months, some morphological responses were noticed as direct shoot buds, direct embryogenesis and callus. Depending on these responses, different culture media with different combinations of auxin and cytokinin were used to differentiate and multiply these cultures. Results indicated that the highest direct shoot bud percentage was formed on M1 medium while the highest direct embryo percentage was formed on M2.Induced direct shoot buds that transferred to the half strength of 2,4D then transferred to auxin free medium showed the highest multiplied rate as clusters. Maximum shoot number was obtained when transferring these clusters to a modified medium supplemented with 3/4 MS salt + 2.0 mg/l BA+ 0.5 mg/l Kinetin+ 0.25 mg/l 2iP+ 1.0 mg/l IAA. Meanwhile, induced direct embryos transferred to a modified medium supplemented with 1/2 MS+ 0.25 mg/l ABA + 0.5 mg/l Kinetin+ 0.25 mg/l 2iP showed the highest secondary embryo number. All induced shoots were rooted successfully and transferred to the greenhouse. | ||
| Keywords | ||
| Date Palm; Direct Organogenesis; Direct Embryogenesis | ||
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